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Literature summary for 3.4.21.9 extracted from

  • Magee, A.I.; Grant, D.A.W.; Hermon-Taylor, J.; Offord, R.E.
    Specific one-stage method for assay of enterokinase activity by release of radiolabelled activation peptides from alpha-N-[3H]acetyl-trypsinogen and the effect of calcium ions on the enzyme activity (1981), Biochem. J., 197, 239-244.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
novel assay method for detection of approximately 1 fmol of enzyme. The method depends on quantification of the release of specifically radiolabelled activation peptides from bovine trypsinogen. The assay is applicable to biological fluids such as serum Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypsinogen + H2O
-
Homo sapiens trypsin + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in presence of 7.5 mM CaCl2 Homo sapiens