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Literature summary for 3.4.21.9 extracted from

  • Azhar, M.; Somashekhar, R.
    Production and purification of recombinant enteropeptidase expressed in an insect-baculovirus cell system (2015), Prep. Biochem. Biotechnol., 45, 268-278.
    View publication on PubMed
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf-9 insect cells Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 Gly-(L-Asp)4-L-Lys-2-naphthylamide pH and temperature not specified in the publication Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypsinogen + H2O Bos taurus
-
 trypsin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus R4QR01 catalytic subunit; subspecies Bos taurus indicus
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-(L-Asp)4-L-Lys-2-naphthylamide + H2O
-
 Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
trypsinogen + H2O
-
 Bos taurus trypsin + ?
-
 ?

General Information

General Information Comment Organism
physiological function the enzyme triggers the activation of the zymogens in pancreatic juice by converting trypsinogen into trypsin Bos taurus