Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) and on M13 filamentous bacteriophage | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Soybean trypsin inhibitor | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
GDDDDK-2-naphthylamide | phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens | |
0.02 | - |
GDDDDK-2-naphthylamide | soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, enteropeptidase catalytic subunit, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | Homo sapiens | recognition sequence (Asp)4-Lys | trypsin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDDDDK-2-naphthylamide + H2O | - |
Homo sapiens | GDDDDK + 2-naphthylamine | - |
? | |
trypsinogen + H2O | recognition sequence (Asp)4-Lys | Homo sapiens | trypsin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, enteropeptidase catalytic subunit, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-HEP | enteropeptidase catalytic subunit | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
92 | - |
GDDDDK-2-naphthylamide | soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens | |
115 | - |
GDDDDK-2-naphthylamide | phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4600 | - |
GDDDDK-2-naphthylamide | soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens | |
6052 | - |
GDDDDK-2-naphthylamide | phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C | Homo sapiens |