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Literature summary for 3.4.21.9 extracted from

  • Gasparian, M.E.; Bobik, T.V.; Kim, Y.V.; Ponomarenko, N.A.; Dolgikh, D.A.; Gabibov, A.G.; Kirpichnikov, M.P.
    Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase (2013), Biochimie, 95, 2076-2081.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) and on M13 filamentous bacteriophage Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Soybean trypsin inhibitor
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
GDDDDK-2-naphthylamide phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens
0.02
-
GDDDDK-2-naphthylamide soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, enteropeptidase catalytic subunit, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypsinogen + H2O Homo sapiens recognition sequence (Asp)4-Lys trypsin + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDDDDK-2-naphthylamide + H2O
-
Homo sapiens GDDDDK + 2-naphthylamine
-
?
trypsinogen + H2O recognition sequence (Asp)4-Lys Homo sapiens trypsin + ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, enteropeptidase catalytic subunit, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
L-HEP enteropeptidase catalytic subunit Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
92
-
GDDDDK-2-naphthylamide soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens
115
-
GDDDDK-2-naphthylamide phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4600
-
GDDDDK-2-naphthylamide soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens
6052
-
GDDDDK-2-naphthylamide phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C Homo sapiens