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Literature summary for 3.4.21.9 extracted from
- Development of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for production of N-terminal methionine-free interferons (2011), Mol. Biol., 45, 466-471.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the enzyme is used for cleavage of the N-terminal part of recombinant human interferon-alpha2a (IFN-alpha2a) and IFN-alpha2b, expressed in Escherichia coli strains strains BL21 and BL21 (DE3), for production of the protein without the N-terminal methionine residue | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trypsinogen + H2O | Sus scrofa | - |
trypsin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from duodenal mucosa by anion exchange and benzoamidine affinity chromatography, followed by hydrophobic interaction chromatography and gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| duodenum | mucosa | Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 330 | - |
purified native enzyme, pH 5.0, 25°C | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| interferon-alpha2a + H2O | recombinantly expressed substrate | Sus scrofa | ? | - |
? | |
| interferon-alpha2b + H2O | recombinantly expressed substrate | Sus scrofa | ? | - |
? | |
| trypsinogen + H2O | - |
Sus scrofa | trypsin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at, substrate trypsin | Sus scrofa |
| 8 | - |
assay at, substrate human interferon-alpha2a | Sus scrofa |
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Release 2023.1 (January 2023)
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