Application | Comment | Organism |
---|---|---|
synthesis | a huge number of therapeutic proteins such as antibodies, coagulation factors, growth hormones or vaccines are produced as fusion proteins. To obtain the therapeutic protein in its monomeric, active form, the fusion partner has to be removed either by chemical or enzymatic cleavage. Enterokinase is a very attractive tool for the in vitro cleavage of fusion proteins | Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ΓKTA-purifier system and a Sepharose QFF column (HiPrep 16/10 QFF) | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O | - |
Bos taurus | Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine | - |
? | |
mucin 1-IgG2a Fc + H2O | cleavage of the mucin fusion protein | Bos taurus | mucin 1 + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enterokinase | - |
Bos taurus |