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Literature summary for 3.4.21.9 extracted from

  • Shahravan, S.H.; Qu, X.; Chan, I.S.; Shin, J.A.
    Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag (2008), Protein Expr. Purif., 59, 314-319.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information changing pH, temperature, or the amount of the enzyme has no effect on cleavage pattern synthetic construct
Urea addition of urea (1-4 M) greatly improves enterokinase cleavage specificity at the canonical site and reduces adventitious cleavage synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
Urea use of higher amounts of urea (ca. 5 M) has an inhibitory effect on the enzyme activity synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AhR6-C/EBP hybrid of the AhR basic region and C/EBP leucine zipper. Reaction under standard conditions (at 37°C in 50 mM Tris buffer, pH 7.6) is rapid and somewhat nonspecific. After just 15 min incubation, 82% of the protein has been cleaved at multiple sites, prolonged incubation causes further degradation of cleavage products synthetic construct ?
-
?

Synonyms

Synonyms Comment Organism
enterokinase
-
synthetic construct