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Literature summary for 3.4.21.9 extracted from

  • Kim, Y.T.; Nishii, W.; Matsushima, M.; Inoue, H.; Ito, H.; Park, S.J.; Takahashi, K.
    Substrate specificities of porcine and bovine enteropeptidases toward the peptide Val-(Asp)4-Lys-Ile-Val-Gly and its analogs (2008), Biosci. Biotechnol. Biochem., 72, 905-908.
    View publication on PubMed
Search for more literature for 3.4.21.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 Val-(Asp)4-Arg-Ile-Val-Gly
-
 Sus scrofa
0.72
-
 Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly
-
 Sus scrofa
0.76
-
 Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly
-
 Sus scrofa
0.8
-
 Val-(Asp)3-Glu-Lys-Ile-Val-Gly
-
 Sus scrofa
1.01
-
 Val-Ala-(Asp)3-Lys-Ile-Val-Gly
-
 Sus scrofa
1.24
-
 Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
1.25
-
 Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
1.51
-
 Val-(Ala)3-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
1.77
-
 Val-(Asp)4-Lys-Ile-Val-Gly
-
 Sus scrofa
3.39
-
 Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
5.57
-
 Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
8.31
-
 Val-(Asp)3-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
8.77
-
 Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
21.11
-
 Val-Asp-(Ala)3-Lys-Ile-Val-Gly
-
 Sus scrofa

Organism

Organism UniProt Comment Textmining
Bos taurus P98072
-
-
Sus scrofa P98074
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Bos taurus
by gel filtration Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Val-(Asp)2-(Ala)2-Lys-Ile-Val-Gly, Val-Ala-Asp-(Ala)2-Lys-Ile-Val-Gly, Val-(Asp)4-Lys-Ile-Val-Gly, Val-(Asp)4-Ala-Ile-Val-Gly and Val-(Asp)4-Glu-Ile-Val-Gly are not hydrolyzed Bos taurus ?
-
 ?
additional information Val-(Asp)2-(Ala)2-Lys-Ile-Val-Gly, Val-Ala-Asp-(Ala)2-Lys-Ile-Val-Gly, Val-(Asp)4-Lys-Ile-Val-Gly, Val-(Asp)4-Ala-Ile-Val-Gly and Val-(Asp)4-Glu-Ile-Val-Gly are not hydrolyzed Sus scrofa ?
-
 ?
Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly + H2O 107% relative activity Sus scrofa Val-(Ala)2-(Asp)2-Lys + Ile-Val-Gly
-
 ?
Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly + H2O 109.3% relative activity Bos taurus Val-(Ala)2-(Asp)2-Lys + Ile-Val-Gly
-
 ?
Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly + H2O 16.8% relative activity Bos taurus Val-(Ala)2-Asp-Ala-Lys + Ile-Val-Gly
-
 ?
Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly + H2O 21.1% relative activity Sus scrofa Val-(Ala)2-Asp-Ala-Lys + Ile-Val-Gly
-
 ?
Val-(Ala)3-Asp-Lys-Ile-Val-Gly + H2O 75.6% relative activity Bos taurus Val-(Ala)3-Asp-Lys + Ile-Val-Gly
-
 ?
Val-(Ala)3-Asp-Lys-Ile-Val-Gly + H2O 81.2% relative activity Sus scrofa Val-(Ala)3-Asp-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly + H2O 76.1% relative activity Bos taurus Val-(Asp)2-Ala-Asp-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly + H2O 93.1% relative activity Sus scrofa Val-(Asp)2-Ala-Asp-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)3-Ala-Lys-Ile-Val-Gly + H2O 16.3% relative activity Bos taurus Val-(Asp)3-Ala-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)3-Ala-Lys-Ile-Val-Gly + H2O 20.2% relative activity Sus scrofa Val-(Asp)3-Ala-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)3-Glu-Lys-Ile-Val-Gly + H2O 42.5% relative activity Bos taurus Val-(Asp)3-Glu-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)3-Glu-Lys-Ile-Val-Gly + H2O 64.9% relative activity Sus scrofa Val-(Asp)3-Glu-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)4-Arg-Ile-Val-Gly + H2O 115.2% relative activity Bos taurus Val-(Asp)4-Arg + Ile-Val-Gly
-
 ?
Val-(Asp)4-Arg-Ile-Val-Gly + H2O 128.2% relative activity Sus scrofa Val-(Asp)4-Arg + Ile-Val-Gly
-
 ?
Val-(Asp)4-Lys-Ile-Val-Gly + H2O 100% relative activity Bos taurus Val-(Asp)4-Lys + Ile-Val-Gly
-
 ?
Val-(Asp)4-Lys-Ile-Val-Gly + H2O 100% relative activity Sus scrofa Val-(Asp)4-Lys + Ile-Val-Gly
-
 ?
Val-Ala-(Asp)2-Ala-Lys-Ile-Val-Gly + H2O 4.3% relative activity Bos taurus Val-Ala-(Asp)2-Ala-Lys + Ile-Val-Gly
-
 ?
Val-Ala-(Asp)2-Ala-Lys-Ile-Val-Gly + H2O 5.6% relative activity Sus scrofa Val-Ala-(Asp)2-Ala-Lys + Ile-Val-Gly
-
 ?
Val-Ala-(Asp)3-Lys-Ile-Val-Gly + H2O 104.2% relative activity Bos taurus Val-Ala-(Asp)3-Lys + Ile-Val-Gly
-
 ?
Val-Ala-(Asp)3-Lys-Ile-Val-Gly + H2O 123.7% relative activity Sus scrofa Val-Ala-(Asp)3-Lys + Ile-Val-Gly
-
 ?
Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly + H2O 35.8% relative activity Sus scrofa Val-Ala-Asp-Ala-Asp-Lys + Ile-Val-Gly
-
 ?
Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly + H2O 48.1% relative activity Bos taurus Val-Ala-Asp-Ala-Asp-Lys + Ile-Val-Gly
-
 ?
Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly + H2O 82.6% relative activity Bos taurus Val-Asp-(Ala)2-Asp-Lys + Ile-Val-Gly
-
 ?
Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly + H2O 95.8% relative activity Sus scrofa Val-Asp-(Ala)2-Asp-Lys + Ile-Val-Gly
-
 ?
Val-Asp-(Ala)3-Lys-Ile-Val-Gly + H2O 6.6% relative activity Sus scrofa Val-Asp-(Ala)3-Lys + Ile-Val-Gly
-
 ?
Val-Asp-(Ala)3-Lys-Ile-Val-Gly + H2O 7.1% relative activity Bos taurus Val-Asp-(Ala)3-Lys + Ile-Val-Gly
-
 ?
Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly + H2O 117.4% relative activity Bos taurus Val-Asp-Ala-(Asp)2-Lys + Ile-Val-Gly
-
 ?
Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly + H2O 119.7% relative activity Sus scrofa Val-Asp-Ala-(Asp)2-Lys + Ile-Val-Gly
-
 ?
Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly + H2O 21.1% relative activity Bos taurus Val-Asp-Ala-Asp-Ala-Lys + Ile-Val-Gly
-
 ?
Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly + H2O 22.5% relative activity Sus scrofa Val-Asp-Ala-Asp-Ala-Lys + Ile-Val-Gly
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 Val-(Asp)3-Glu-Lys-Ile-Val-Gly
-
 Sus scrofa
9.1
-
 Val-Asp-(Ala)3-Lys-Ile-Val-Gly
-
 Sus scrofa
10.3
-
 Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
13.6
-
 Val-(Asp)4-Arg-Ile-Val-Gly
-
 Sus scrofa
14.1
-
 Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
14.3
-
 Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
14.6
-
 Val-(Asp)3-Ala-Lys-Ile-Val-Gly
-
 Sus scrofa
14.7
-
 Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly
-
 Sus scrofa
15.9
-
 Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly
-
 Sus scrofa
17
-
 Val-(Ala)3-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
17.8
-
 Val-Ala-(Asp)3-Lys-Ile-Val-Gly
-
 Sus scrofa
20
-
 Val-(Asp)4-Lys-Ile-Val-Gly
-
 Sus scrofa
23
-
 Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa
28.1
-
 Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 shows pH optima at 8.0-8.5 for Val-(Asp)4-Lys-Ile-Val-Gly and 8.5-9 for Val-(Asp)3-Ala-Lys-Ile-Val-Gly Sus scrofa