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Literature summary for 3.4.21.9 extracted from

  • Gasparian, M.E.; Ostapchenko, V.G.; Dolgikh, D.A.; Kirpichnikov, M.P.
    Biochemical characterization of human enteropeptidase light chain (2006), Biochemistry (Moscow), 71, 113-119.
    View publication on PubMed
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expression as fusion protein with thioredoxin in Escherichia coli Homo sapiens
expression as fusion protein with thioredoxin in Escherichia coli Bos taurus

General Stability

General Stability Organism
highly resistant to detergent to triton X-100, tween 20 and SDS Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antipain 85-95% loss of activtity Homo sapiens
Aprotinin 40-90% loss of activtity Homo sapiens
benzamidine 75-92% loss of activtity Homo sapiens
bestatin 7% loss of activtity Homo sapiens
chymostatin 20% loss of activtity Homo sapiens
dithiothreitol 10% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
E-64 50-85% loss of activtity Homo sapiens
ethanol 80-40% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
imidazole 90-30% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
leupeptin 94% loss of activtity Homo sapiens
additional information EDTA not inhibitory Homo sapiens
N-tosyl-L-lysine chloromethyl ketone 13% loss of activtity Homo sapiens
NaCl at 100 and 250 mM, Gly-Asp-Asp-Asp-Asp-Lys-?-naphthylamide as substrate Homo sapiens
pepstatin 5% loss of activtity Homo sapiens
pepstatin A 5% loss of activtity Homo sapiens
PMSF 50% loss of activtity Homo sapiens
SDS 40-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
Soybean trypsin inhibitor 40-90% loss of activtity Homo sapiens
Triton X-100 30-80% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
Tween-20 20-75% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
Urea 50-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 Homo sapiens
0.067
-
 Z-Ala-Phe-Arg-nitroanilide
-
 Homo sapiens
0.076
-
 N-formyl-Ala-Phe-Arg-4-nitroanilide
-
 Homo sapiens
0.08
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA Homo sapiens
0.091
-
 Z-Gly-Pro-Arg-nitroanilide
-
 Homo sapiens
0.109
-
 Z-Ala-Tyr-Arg-nitroanilide
-
 Homo sapiens
0.14
-
 thiobenzyl benzyloxycarbonyl-L-lysinate
-
 Homo sapiens
0.141
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 Bos taurus
0.167
-
 N-formyl-Ala-Phe-Lys-4-nitroanilide
-
 Homo sapiens
0.234
-
 Z-Ala-Leu-Arg-nitroanilide
-
 Homo sapiens
0.24
-
 Z-Ala-Trp-Arg-nitroanilide
-
 Homo sapiens
0.265
-
 Z-Pro-Phe-Arg-nitroanilide
-
 Homo sapiens
0.332
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA Bos taurus
0.356
-
 Z-Ala-Ala-Arg-nitroanilide
-
 Homo sapiens
0.42
-
 Z-Ala-Met-Arg-nitroanilide
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ optimal at 0.02mM, inhibitory at 10 mM Homo sapiens
Ca2+ optimal at 0.02mM, inhibitory at 10 mM Bos taurus
K+ activating, thiobenzyl benzyloxycarbonyl-L-lysinate as non-specific substrate Homo sapiens
Na+ activating, thiobenzyl benzyloxycarbonyl-L-lysinate as non-specific substrate Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilization of inclusion bodies with 6 M guanidine-HCl, affinity chromatography on STI-agarose Homo sapiens
solubilization of inclusion bodies with 6 M guanidine-HCl, affinity chromatography on STI-agarose Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
 thioredoxin-human epidermal growth factor fusion protein as substrate Bos taurus
2100
-
 thioredoxin/human epidermal growth factor fusion protein as substrate Homo sapiens

Storage Stability

Storage Stability Organism
100% activity during storage at –20°C for at least for 1.5 years Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-alanine-X-L-arginine-p-nitroanilide + H2O Z-Ala-X-Arg-pNA Homo sapiens p-nitroaniline + benzyloxycarbonyl-L-alanine-X-L-arginine
-
 ?
formyl-Ala-Phe-Arg-4-nitroanilide + H2O
-
 Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Arg
-
 ?
formyl-Ala-Phe-Lys-4-nitroanilide + H2O
-
 Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Lys
-
 ?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O GD4K-na Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide + H2O GD4K-na Bos taurus naphthylamine + Gly-Asp-Asp-Asp-Asp-Lys
-
 ?
thiobenzyl benzyloxycarbonyl-L-lysinate + H2O Z-Lys-SBzl Homo sapiens 3-carboxy-4-nitrothiophenoxide + ?
-
 ?
thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Homo sapiens thioredoxin + human epidermal growth factor
-
 ?
thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Bos taurus thioredoxin + human epidermal growth factor
-
 ?
Z-Ala-Ala-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Ala-Arg
-
 ?
Z-Ala-Leu-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Leu-Arg
-
 ?
Z-Ala-Met-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Met-Arg
-
 ?
Z-Ala-Phe-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Phe-Arg
-
 ?
Z-Ala-Trp-Arg-p-nitroanilide + H2O
-
 Homo sapiens Z-Ala-Trp-Arg + 4-nitroaniline
-
 ?
Z-Ala-Tyr-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Tyr-Arg
-
 ?
Z-Gly-Pro-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Gly-Pro-Arg
-
 ?
Z-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Pro-Phe-Arg
-
 ?

Synonyms

Synonyms Comment Organism
enteropeptidase
-
 Homo sapiens
enteropeptidase
-
 Bos taurus
enteropeptidase light chain L-BEP Bos taurus
enteropeptidase light chain L-HEP Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Homo sapiens