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Literature summary for 3.4.21.9 extracted from

  • Nemoda, Z.; Sahin-Toth, M.
    The tetra-aspartate motif in the activation peptide of human cationic trypsinogen is essential for autoactivation control but not for enteropeptidase recognition (2005), J. Biol. Chem., 280, 29645-29652.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
human cationic trypsinogen 37 °C, 100 mM Tris-HCl pH 8.0, 1mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase Homo sapiens
0.0021
-
A4-mutant trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase Homo sapiens
0.0072
-
human cationic trypsinogen 25 °C, 28 mM sodium succinate (pH 5.6), 10 mM CaCl2 Homo sapiens
1.2
-
bovine trypsinogen 37 °C, 25 mM Tris-HCl pH 8.4, 10 mM CaCl2, 0. 4 mM ovomucoid, 0.3 nM enteropeptidase Bos taurus
1.5
-
human cationic trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.45 nM enteropeptidase Bos taurus
5.6
-
bovine trypsinogen 21 °C, 50 mM sodium citrate pH 5.6, 1 nM enteropeptidase Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
A4-mutant trypsinogen + H2O
-
Homo sapiens ?
-
?
bovine trypsinogen + H2O
-
Bos taurus ?
-
?
human cationic trypsinogen + H2O
-
Homo sapiens ?
-
?
human cationic trypsinogen + H2O
-
Bos taurus ?
-
?

Synonyms

Synonyms Comment Organism
bovine enteropeptidase
-
Bos taurus
enterokinase
-
Homo sapiens
enterokinase
-
Bos taurus
human enteropeptidase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
human cationic trypsinogen 25°C, 28 mM sodium succinate (pH 5.6), 10 mM CaCl2 Homo sapiens
2.3
-
human cationic trypsinogen 0.00036 1/sec/mg, incubates 30 min at 37°C, pH 9.0, spectrophotometrically measured at 415 nm Homo sapiens
4
-
bovine trypsinogen 21 °C, 50 mM sodium citrate pH 5.6, 1 nM enteropeptidase Bos taurus
6.9
-
bovine trypsinogen 37 °C, 25 mM Tris-HCl pH 8.4, 10 mM CaCl2, 0. 4 mM ovomucoid, 0.3 nM enteropeptidase Bos taurus
8.8
-
human cationic trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.45 nM enteropeptidase Bos taurus
11.2
-
A4-mutant trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM enteropeptidase Homo sapiens
35.1
-
human cationic trypsinogen 37 °C, 100 mM Tris-HCl pH 8.0, 1mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM enteropeptidase Homo sapiens