Literature summary for 3.4.21.9 extracted from

Yuan, L.D.; Hua, Z.C.
Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli (2002), Protein Expr. Purif., 25, 300-304.

Search for more literature for 3.4.21.9

Application

Application	Comment	Organism
synthesis	useful tool for in vitro cleavage of fusion proteins	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
catalytic subunit is expressed in escherichia coli BL21	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide	pH 8.0, 25°C	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic subunit	Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O	-	Bos taurus	Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20.8	-	Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide	pH 8.0, 25°C	Bos taurus

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Release 2023.1 (January 2023)