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Literature summary for 3.4.21.89 extracted from

  • Carlos, J.L.; Paetzel, M.; Brubaker, G.; Karla, A.; Ashwell, C.M.; Lively, M.O.; Cao, G.; Bullinger, P.; Dalbey, R.E.
    The role of the membrane-spanning domain of type I signal peptidases in substrate cleavage site selection (2000), J. Biol. Chem., 275, 38813-38822.
    View publication on PubMed
Search for more literature for 3.4.21.89

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 strongly stimulated by detergents Escherichia coli

Application

Application Comment Organism
pharmacology possible targets for the design of novel antibiotics Escherichia coli
pharmacology possible targets for the design of novel antibiotics Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
cloning of the WT and P2 domain of SipS, MBP-SipS fusion protein expressed in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0166
-
 preprotein substrate PONA pH 8.0, 37°C, A14PONA mutant, SipS Bacillus subtilis
0.0192
-
 preprotein substrate PONA pH 8.0, 37°C, Lep Escherichia coli
0.0425
-
 preprotein substrate PONA pH 8.0, 37°C, A14PONA mutant, Lep Escherichia coli
0.044
-
 preprotein substrate PONA pH 8.0, 37°C, SipS Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 recombinant fusion protein MBP-SipS-P2 Escherichia coli
60000
-
 recombinant fusion protein MBP-SipS-P2 Bacillus subtilis
63000
-
 recombinant fusion protein MBP-SipS Escherichia coli
63000
-
 recombinant fusion protein MBP-SipS Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Escherichia coli
-
 mature proteins
-
 ?
signal peptides from preproteins + H2O Bacillus subtilis
-
 mature proteins
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P28628
-
-
Escherichia coli P00803
-
-

Purification (Commentary)

Purification (Comment) Organism
Lep and mutant delta2-75 Escherichia coli
MBP-SipS fusion protein, SipS, SipS-P2 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
preprotein substrate PONA + H2O
-
 Escherichia coli protein substrate PONA + ?
-
 ?
preprotein substrate PONA + H2O
-
 Bacillus subtilis protein substrate PONA + ?
-
 ?
pro-ompA-nuclease + H2O
-
 Escherichia coli ompA-nuclease + ?
-
 ?
signal peptides from preproteins + H2O
-
 Escherichia coli mature proteins
-
 ?
signal peptides from preproteins + H2O
-
 Bacillus subtilis mature proteins
-
 ?

Synonyms

Synonyms Comment Organism
LEP
-
 Escherichia coli
SipP (pTA1015) plasmid-encodes type I signal peptidase Bacillus subtilis
SipP (pTA1040) plasmid-encodes type I signal peptidase Bacillus subtilis
SipS chromsomal type I signal peptidase Bacillus subtilis
SipT chromsomal type I signal peptidase Bacillus subtilis
SipU chromsomal type I signal peptidase Bacillus subtilis
SipV chromsomal type I signal peptidase Bacillus subtilis
SipW chromsomal type I signal peptidase Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 preprotein substrate PONA pH 8.0, 37°C, A14PONA mutant, SipS Bacillus subtilis
0.034
-
 preprotein substrate PONA pH 8.0, 37°C, SipS Bacillus subtilis
0.043
-
 preprotein substrate PONA pH 8.0, 37°C, A14PONA mutant, Lep Escherichia coli
44
-
 preprotein substrate PONA pH 8.0, 37°C, Lep Escherichia coli