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Literature summary for 3.4.21.89 extracted from

  • Ng, S.Y.M.; Jarrell, K.F.
    Cloning and characterization of archael type I signal peptidase from Methanococcus voltae (2003), J. Bacteriol., 185, 5936-5942.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli BL21(DE3)/pLysS with pKJ341 and in Escherichia coli BL21(DE3) harboring pSJS1240 Methanococcus voltae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane archaeal enzyme cleaves the signal peptides from preproteins on the external face of the cytoplasmic membrane, thereby releasing the translocated protein from the membrane Methanococcus voltae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
his-tagged version, overexpressed in Escherichia coli, Western blotting Methanococcus voltae
24600
-
deduced from DNA sequence Methanococcus voltae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Methanococcus voltae
-
mature proteins
-
?
signal peptides from preproteins + H2O Methanococcus voltae PS
-
mature proteins
-
?

Organism

Organism UniProt Comment Textmining
Methanococcus voltae
-
-
-
Methanococcus voltae PS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Methanococcus voltae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Methanococcus voltae S-layer protein + H2O truncated, his-tagged form Methanococcus voltae ?
-
?
Methanococcus voltae S-layer protein + H2O truncated, his-tagged form Methanococcus voltae PS ?
-
?
signal peptides from preproteins + H2O
-
Methanococcus voltae mature proteins
-
?
signal peptides from preproteins + H2O
-
Methanococcus voltae PS mature proteins
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
Methanococcus voltae