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Literature summary for 3.4.21.89 extracted from
- Kinetic constants of signal peptidase I using cytochrome b5 as a precursor substrate (2001), Biochim. Biophys. Acta, 1550, 1-5.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | pH 7.5, 37°C | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | integral component of the cytoplasmic membrane | Escherichia coli | 5737 | - |
| plasma membrane | integral membrane protein | Escherichia coli | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| signal peptides from preproteins + H2O | Escherichia coli | in vivo, type I signal peptidase is the principal peptidase responsible for signal peptide cleavage as pre-proteins of a number of exported proteins, proteins designed for transport across the cytoplasmic membrane are generally synthesised as precursors with cleavable signal peptides in the cytoplasm, the signal peptides targets the pre-proteins to the respective translocase, during or shortly after translocation across the cytoplasmic membrane, the signal peptide is enzymatically removed | mature proteins | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | amphipatic, chimeric cytochrome b5 precursor | Escherichia coli | cytochrome b5 | - |
? | |
| pro-ompA-nuclease + H2O | - |
Escherichia coli | ompA-nuclease + ? | - |
? | |
| signal peptides from preproteins + H2O | - |
Escherichia coli | mature proteins | - |
? | |
| signal peptides from preproteins + H2O | in vivo, type I signal peptidase is the principal peptidase responsible for signal peptide cleavage as pre-proteins of a number of exported proteins, proteins designed for transport across the cytoplasmic membrane are generally synthesised as precursors with cleavable signal peptides in the cytoplasm, the signal peptides targets the pre-proteins to the respective translocase, during or shortly after translocation across the cytoplasmic membrane, the signal peptide is enzymatically removed | Escherichia coli | mature proteins | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| signal peptidase I | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5 | pH 7.5, 37°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | maximal processing rates at pH 8.5 and above, 90% activity at neutral pH | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | 20% of maximal activity at pH 4.5, half-maximal activity at pH 6.5, 90% of maximal activity at pH 7.0 | Escherichia coli |
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