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Literature summary for 3.4.21.88 extracted from

  • Kuchinski, K.S.; Brimacombe, C.A.; Westbye, A.B.; Ding, H.; Beatty, J.T.
    The SOS response master regulator LexA regulates the gene transfer agent of Rhodobacter capsulatus and represses transcription of the signal transduction protein CckA (2016), J. Bacteriol., 198, 1137-1148 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.88

Cloned(Commentary)

Cloned (Comment) Organism
gene lexA, construction of complementing plasmid pLexA from the rcc02361 coding region and flanking sequences are amplified from SB1003 genomic DNA, digested, and cloned into plasmid pCM62 Rhodobacter capsulatus

Protein Variants

Protein Variants Comment Organism
additional information construction of knockout mutant DELTAlexA mutant, the decrease in gene transfer frequency from the DELTAlexA mutant is associated with a decrease in transcription of the RcGTA structural gene cluster, the -galactosidase activities of WTand DELTAlexA mutant cells containing a plasmid with the RcGTA promoter fused to lacZ are compared. RcGTA recipient capability is decreased in the DELTAlexA mutant, phenotype, and morphology of DELTAlexA mutant cells, overview Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodobacter capsulatus Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA ?
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additional information Rhodobacter capsulatus NBRC 16581 Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA ?
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additional information Rhodobacter capsulatus ATCC BAA-309 Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA ?
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additional information Rhodobacter capsulatus SB1003 Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA ?
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Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus D5ALN0
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Rhodobacter capsulatus ATCC BAA-309 D5ALN0
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Rhodobacter capsulatus NBRC 16581 D5ALN0
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Rhodobacter capsulatus SB1003 D5ALN0
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA Rhodobacter capsulatus ?
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additional information Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA Rhodobacter capsulatus NBRC 16581 ?
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additional information Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA Rhodobacter capsulatus ATCC BAA-309 ?
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 ?
additional information Rhodobacter capsulatus LexA binds to the predicted promoter regions of lexA, recA, and cckA and represses cckA and recA transcription. Putative LexA binding sites are present 5' of SOS response gene homologues and the RcGTA regulatory gene cckA Rhodobacter capsulatus SB1003 ?
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General Information

General Information Comment Organism
malfunction deletion of the lexA gene results in the abolition of detectable RcGTA production and an about 10fold reduction in recipient capability. Expression of gene cckA, encoding the sensor kinase CckA, is increased over 5fold in the lexA mutant, and a lexA/cckA double mutant is found to have the same phenotype as a DELTAcckA single mutant in terms of RcGTA production. The RcGTA-deficient phenotype of the lexA mutant is largely due to the overexpression of cckA. RcGTA transposon Tn5 mutagenesis, overview Rhodobacter capsulatus
metabolism LexA-mediated repression of recA expression is a hallmark of the prototypical SOS response Rhodobacter capsulatus
additional information identification of putative Lex binding sites located 5' of typical SOS response coding sequences and also 5' of the RcGTA regulatory gene cckA, which encodes a hybrid histidine kinase homologue Rhodobacter capsulatus
physiological function the LexA repressor, the master regulator of the SOS response in many bacteria, as a regulator of RcGTA activity. Gene transfer agent of Rhodobacter capsulatus (RcGTA) is a genetic exchange element that combines central aspects of bacteriophage-mediated transduction and natural transformation. RcGTA particles resemble a small double-stranded DNA bacteriophage, package random about 4-kb fragments of the producing cell genome, and are released from a subpopulation (below 1%) of cells in a stationary-phase culture. RcGTA particles deliver this DNA to surrounding Rhodobacter capsulatus cells, and the DNA is integrated into the recipient genome though a process that requires homologs of natural transformation genes and RecA-mediated homologous recombination. LexA is required for RcGTA production and maximal recipient capability, the regulation of RcGTA production is complex and involves several bacterial regulatory factors Rhodobacter capsulatus