Literature summary for 3.4.21.83 extracted from

Motta, F.; Bastos, I.; Faudry, E.; Ebel, C.; Lima, M.; Neves, D.; Ragno, M.; Barbosa, J.; de Freitas, S.; Santana, J.
The Trypanosoma cruzi virulence factor oligopeptidase B (OPBTc) assembles into an active and stable dimer (2012), PLoS ONE, 7, e30431.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	dithiothreitol is not inhibitoryup to 40 mM	Trypanosoma cruzi
NaCl	loss of almost 50 and 70% of its activity in the presence of 0.2 and 1.0 M NaCl, respectively. Presence of NaCl does not disrupt the dimeric structure	Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	2 * 80000, SDS-PAGE	Trypanosoma cruzi
120000	-	PAGE	Trypanosoma cruzi
189000	-	gel filtration	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-
Trypanosoma cruzi Brener	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O	-	Trypanosoma cruzi	benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O	-	Trypanosoma cruzi Brener	benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 80000, SDS-PAGE	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
OPBTc	-	Trypanosoma cruzi

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
42	-	enzyme retains its dimeric structure and is fully active up to 42°C	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Trypanosoma cruzi

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	-	about 80% of maximum activity	Trypanosoma cruzi

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Release 2023.1 (January 2023)