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Literature summary for 3.4.21.83 extracted from

  • Mikhailova, A.G.; Khairullin, R.F.; Demidyuk, I.V.; Gromova, T.Y.; Kostrov, S.V.; Rumsh, L.D.
    Oligopeptidase B from Serratia proteamaculans. II. Enzymatic characteristics: substrate analysis, influence of calcium ions, pH and temperature dependences (2011), Biochemistry (Moscow), 76, 480-490.
    View publication on PubMed
Search for more literature for 3.4.21.83

Inhibitors

Inhibitors Comment Organism Structure
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
 Serratia proteamaculans
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
 Serratia proteamaculans
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
 Serratia proteamaculans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0226
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.0513
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.058
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.084
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.09
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.096
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
0.224
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.265
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.302
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.306
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.322
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.457
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.54
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
1.05
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
2.2
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in presence of Ca2+, efficiency of hydrolysis of substrates with positively charged P2 residues decreases by an order of magnitude due to the worsening in the substrate binding. In the presence of 50 mM Ca2+, no substrate inhibition is observed in the case of acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide, and the effect was less pronounced for other substrates Serratia proteamaculans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 x * 78000, SDS-PAGE Serratia proteamaculans

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide assay in presence of 2% dimethyl sulfoxide Serratia proteamaculans

Organism

Organism UniProt Comment Textmining
Serratia proteamaculans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline
-
 ?
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline
-
 ?
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Serratia proteamaculans Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O poor substrate Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline
-
 ?
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O
-
 Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol
-
 ?
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
-
 ?
succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O poor substrate Serratia proteamaculans succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
-
 Serratia proteamaculans succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
-
 ?
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Serratia proteamaculans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Serratia proteamaculans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 rapid loss of activity above Serratia proteamaculans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
1
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
1.2
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
2 8 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
7.6
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
13.9
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
14.5
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
21.2
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
24.3
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
32
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
35.2
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
37.7
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
53.8
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
55.5
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
201.7
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Serratia proteamaculans

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
 Serratia proteamaculans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.027
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.045
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.126
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.19
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.4
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
0.4
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
0.5
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
2
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
5.8
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
10.8
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
30
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
34
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
120
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
125
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
161
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
251
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
377
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
485
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
680
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans