Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | enzymatic activity is markedly enhanced by dithiothreitol at submillimolar level, which affects the P1 preference of OpdB synergistically in a substrate-dependent manner | Streptomyces griseus | |
NaCl | enzymatic activity is markedly enhanced by high NaCl concentration, NaCl affects the P1 preference of OpdB synergistically in a substrate-dependent manner | Streptomyces griseus | |
oxidized glutathione | oxidized glutathione enhances OpdB activity and affects the P1 preference of OpdB synergistically in a substrate-dependent manner | Streptomyces griseus | |
reduced glutathione | enzymatic activity is markedly enhanced by reduced glutathione at submillimolar level which affects the P1 preference of OpdB synergistically in a substrate-dependent manner | Streptomyces griseus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
C544A | the enzymatic activity of the Cys-free mutant is markedly activated by the general thiol-reacting reagent dithiothreitol, oxidized glutathione, and reduced glutathione,the mutant is insensitive to the treatment of the SH-blocking reagent N-ethylmaleimide or H2O2 | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride | OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 4 mM pefbloc SC | Streptomyces griseus | |
antipain | OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.3 mM antipain | Streptomyces griseus | |
leupeptin | OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.05 mM leupeptin | Streptomyces griseus | |
additional information | OpdB is not inhibited by E-64 (0.03 mM), pepstain (0.04 mM), EDTA (1 mM), chimostatin (0.2 mM), and phosphoramidon (0.4 mM) | Streptomyces griseus | |
N-ethylmaleimide | - |
Streptomyces griseus | |
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | in the case of added dithiothreitol, reduced glutathione, or oxidized glutathione, a high concentration of the substrate tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin inhibits the enzyme activity. By contrast, addition of 1 M NaCl to either dithiothreitol, reduced glutathione, or oxidized glutathione or 1 M NaCl alone prevents substrate inhibition | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O | weak substrate | Streptomyces griseus | CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-pGlu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O | low hydrolytic rate | Streptomyces griseus | L-pGlu-Gly-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O | low hydrolytic rate | Streptomyces griseus | L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | no hydrolytic activity against the aminopeptidase substrates (4-nitroanilide derivatives of amino acids) and FITC-casein, substrates containing Pro at Xaa positions in the FRETS library are not hydrolyzed | Streptomyces griseus | ? | - |
? | |
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | best substrate, the hydrolytic activity is observed only in the presence of NaCl and the thiol-reacting reagents dithiothreitol, reduced glutathione, and oxidized glutathione at pH 8.5 (50 mM Tris-HCl) | Streptomyces griseus | tert-butoxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O | - |
Streptomyces griseus | tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oligopeptidase B | - |
Streptomyces griseus |
OpdB | - |
Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
pH optimum in the presence of NaCl | Streptomyces griseus |
8.8 | - |
pH optimum in the absence of NaCl | Streptomyces griseus |