Literature summary for 3.4.21.83 extracted from

Morty, R.E.; Shih, A.Y.; Fulop, V.; Andrews, N.W.
Identification of the reactive cysteine residues in oligopeptidase B from Trypanosoma brucei (2005), FEBS Lett., 579, 2191-2196.

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Protein Variants

Protein Variants	Comment	Organism
C256S	750fold reduction in inhibition rate constant for iodoacetic acid and more than 100fold reduction in inhibition rate constant for NEM compared to wild-type enzyme	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	-	Trypanosoma brucei
NEM	Cys256 is the reactive cysteine residue that mediates OpdB inhibition. Cys256 adducts occlude the P1 substrate-binding site, preventing substrate binding	Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	x * 80000, SDS-PAGE under reducing and non-reducing conditions	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O	-	Trypanosoma brucei	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 80000, SDS-PAGE under reducing and non-reducing conditions	Trypanosoma brucei

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Release 2023.1 (January 2023)