Any feedback?
Literature summary for 3.4.21.83 extracted from
- Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme (1991), J. Biochem., 110, 315-320.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Escherichia coli |  |
| DFP | - |
Escherichia coli | |
| leupeptin | weak | Escherichia coli | |
| tosyl-Leu chloromethyl ketone | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
benzoyl-Arg 2-naphthylamide | - |
Escherichia coli | |
| 0.92 | - |
Benzoyl-Lys 2-naphthylamide | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ser532 is the reactive serine residue | Escherichia coli |
| 80000 | - |
1 * 80000, SDS-PAGE without mercaptoethanol | Escherichia coli |
| 81000 | - |
E. coli, gel filtration | Escherichia coli |
| 81860 | - |
E. coli, calculation from nucleotide sequence | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
HB101 gene cloned and expressed in JM83 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HB101 gene cloned and expressed in JM83 | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Lys 2-naphthylamide + H2O | - |
Escherichia coli | Benzoyl-Lys + 2-naphthylamine | - |
? | |
| Insulin B-chain + H2O | cleavage at the carboxyl sides of Arg and Lys residues | Escherichia coli | ? | - |
? | |
| N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O | weak | Escherichia coli | N-Benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
| Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Escherichia coli | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE without mercaptoethanol | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
30 min, pH 8.0, 50% loss of activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
benzoyl-Arg 2-naphthylamide | - |
Escherichia coli | |
| 4.4 | - |
Benzoyl-Lys 2-naphthylamide | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.2 | Escherichia coli |
| 8 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | stable | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
