Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DFP | - |
Streptomyces griseus | |
additional information | metal chelators; metals including Hg2+; not: EDTA; SH-blocking agents | Streptomyces griseus | |
PCMB | partial | Streptomyces griseus | |
tosylphenylalanine chloromethyl ketone | partial | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
Streptomyces griseus, gel filtration | Streptomyces griseus |
22000 | - |
1 * 22000, Streptomyces griseus, SDS-PAGE in presence of 2-mercaptoethanol | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | pronase | Streptomyces griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
137 | - |
- |
Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | preferentially hydrolyzes peptide bonds on the carbonyl-terminal side of either glutamic acid or aspartic acid | Streptomyces griseus | ? | - |
? | |
tert-butoxycarbonyl-Ala-Ala-Pro-Asp 4-nitroanilide + H2O | slowly | Streptomyces griseus | tert-butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline | - |
? | |
tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O | - |
Streptomyces griseus | tert-butoxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline | - |
? | |
tert-butoxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O | - |
Streptomyces griseus | tert-butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22000, Streptomyces griseus, SDS-PAGE in presence of 2-mercaptoethanol | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 8.4 | Streptomyces griseus |
8.8 | - |
tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide | Streptomyces griseus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | relatively stable | Streptomyces griseus |