Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.81 extracted from

  • Qasim, M.A.; Song, J.; Markley, J.L.; Laskowski, M.
    Cleavage of peptide bonds bearing ionizable amino acids at P(1) by serine proteases with hydrophobic S(1) pocket (2010), Biochem. Biophys. Res. Commun., 400, 507-510.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.81

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from a commercial preparation of Pronase Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide + H2O extremely poor substrate at pH 8 but becomes a moderately good substrate at pH 5.5 Streptomyces griseus succinyl-L-Ala-L-Ala-L-Pro-L-Asp + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O optimum at pH 7.5-8.0 Streptomyces griseus succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O optimum at pH 10.0 Streptomyces griseus succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 substrate succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide Streptomyces griseus
7.5 8 substrate succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide Streptomyces griseus
10
-
 substrate succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide Streptomyces griseus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
740
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide pH 8.0, 22°C Streptomyces griseus
10700
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide pH 4.0, 22°C Streptomyces griseus
28200
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide pH 4.0, 22°C Streptomyces griseus
25000000
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide pH 8.0, 22°C Streptomyces griseus