Cloned (Comment) | Organism |
---|---|
ectopic expression of granzyme B-epidermal growth factor receptor peptide ligand transforming growth factor alpha chimeric enzyme GrB-TGFalpha in natural killer cells from a lentiviral vector, the fusion enzyme shows enhanced natural cytotoxicity and increased specific killing of tumor cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a recombinant chimeric enzyme by fusion of the pre-pro-granzyme B to the epidermal growth factor receptor peptide ligand transforming growth factor alpha. Activation of the genetically modified natural killer cells by cognate target cells resulted in the release of chimeric enzyme GrB-TGFalpha together with endogenous granzymes and perforin, which augments the effector cells natural cytotoxicity against NK-sensitive tumor cells. The chimeric enzyme GrB-TGFalpha is released into the extracellular space upon induction of degranulation with phspbol-12-myristate-13-acetate and ionomycin. The secreted GrB-TGFalpha chimeric enzyme displays specific binding to EGFR-overexpressing tumor cells, enzymatic activity, and selective target cell killing in the presence of an endosomolytic activity. Release of GrB-TGFalpha protein from NKL/GrB-TGFalpha cells upon activation-induced degranulation | Homo sapiens |
S183A | inactive mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, recombinnat chimeric enzyme GrB-TGFalpha, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P10144 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
natural killer cell | - |
Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 40000, recombinnat chimeric enzyme GrB-TGFalpha, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
granzyme B | - |
Homo sapiens |
GrB | - |
Homo sapiens |
pro-apoptotic serine protease | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | natural killer cells exhibit cell killing activity that primarily mediated by the pro-apoptotic serine protease granzyme B, which enters targets cells with the help of the pore-forming protein perforin | Homo sapiens |