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Literature summary for 3.4.21.79 extracted from

  • Casciola-Rosen, L.; Garcia-Calvo, M.; Bull, H.G.; Becker, J.W.; Hines, T.; Thornberry, N.A.; Rosen, A.
    Mouse and human granzyme B have distinct tetrapeptide specificities and abilities to recruit the bid pathway (2007), J. Biol. Chem., 282, 4545-4552.
    View publication on PubMed
Search for more literature for 3.4.21.79

Application

Application Comment Organism
analysis caution in the design and interpretation of experiments using GrBs from different species due to distinct tetrapeptide specificities and abilities to recruit the BID pathway Mus musculus
analysis caution in the design and interpretation of experiments using GrBs from different species due to distinct tetrapeptide specificities and abilities to recruit the BID pathway Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant GrB using a baculovirus expression system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid peptide-mimetic analog of IEPD-aldehyde Homo sapiens
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid peptide-mimetic analog of IEPD-aldehyde Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
BID + H2O Homo sapiens efficiently cleaves both human or mouse BID truncated BID + ?
-
 ?
additional information Mus musculus both human or mouse BID are highly resistant to cleavage by mouse GrB ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expressed in Pichia pastoris Mus musculus
to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-IETD-7-amido-4-methylcoumarin + H2O
-
 Mus musculus acetyl-IETD + 7-amino-4-methylcoumarin
-
 ?
acetyl-IETD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens acetyl-IETD + 7-amino-4-methylcoumarin
-
 ?
BID + H2O efficiently cleaves both human or mouse BID Homo sapiens truncated BID + ?
-
 ?
additional information both human or mouse BID are highly resistant to cleavage by mouse GrB Mus musculus ?
-
 ?
additional information both human or mouse BID are highly resistant to cleavage by mouse GrB. It is unable to cleave BID in cell lysates in which caspases are inactivated but induces BID cleavage, albeit inefficiently, in lysates supporting caspase activity. BID pathway is not a prominent primary mediator of the effects of mouse GrB Mus musculus ?
-
 ?
pro-caspase-3 + H2O cleaves human pro-caspase-3 but only generates a 21-kDa fragment, no autocatalytic removal of the caspase-3 prodomain. Efficiently cleaves and activates mouse procaspase-3, producing predominantly the 17-kDa fragment Mus musculus caspase-3 + ?
-
 ?
pro-caspase-3 + H2O cleaves human pro-caspase-3 generating a 21-kDa and a 17-kDa fragment (autocatalytic removal of the caspase-3 prodomain) Homo sapiens caspase-3 + ?
-
 ?

Synonyms

Synonyms Comment Organism
GrB
-
 Mus musculus
GrB
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000008
-
 (S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid
-
 Homo sapiens
0.000025
-
 (S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid
-
 Mus musculus