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Literature summary for 3.4.21.79 extracted from

  • Sun, J.; Bird, C.H.; Thia, K.Y.; Matthews, A.Y.; Trapani, J.A.; Bird, P.I.
    Granzyme B encoded by the commonly occurring human RAH allele retains pro-apoptotic activity (2004), J. Biol. Chem., 279, 16907-16911.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Q48R/P88A/Y245H common allele termed RAH, mutant enzmye has essentially identical proteolytic and cytotoxic properties to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
Abz-IEPDSSMESK-DNP mutant Q48R/P88A/Y245H, 37°C Homo sapiens
0.0058
-
Abz-IEPDSSMESK-DNP wild-type, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
encoded by RAH allele
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
native wild-type, 37°C Homo sapiens
82
-
recombinant wild-type, 37°C Homo sapiens
131
-
mutant Q48R/P88A/Y245H, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-IEPDSSMESK-2,4-dinitrophenyl + H2O
-
Homo sapiens ?
-
?
Abz-IEPDSSMESK-DNP + H2O
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
Abz-IEPDSSMESK-2,4-dinitrophenyl wild-type, 37°C Homo sapiens
4.6
-
Abz-IEPDSSMESK-2,4-dinitrophenyl mutant Q48R/P88A/Y245H, 37°C Homo sapiens