Protein Variants | Comment | Organism |
---|---|---|
S183A | no catalytic activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.16 | - |
acetyl-IETD-p-nitroanilide | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
7.38 | - |
acetyl-IETD-p-nitroanilide | native enzyme, pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | rapid uptake of external enzyme by HeLa cells and accumulation in cytoplasm in inactive form, uptake does not depend on enzyme activity or on mannose 6-phosphate receptor | Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | after prolonged incubation, enzyme causes pronounced morphological changes in human tumor cells, leading to partial loss of contact to the culture support | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
expression in Pichia pastoris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-IETD-p-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | after prolonged incubation, enzyme causes pronounced morphological changes in human tumor cells, leading to partial loss of contact to the culture support | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.34 | - |
acetyl-IETD-p-nitroanilide | native enzyme, pH 7.4, 37°C | Homo sapiens | |
5.23 | - |
acetyl-IETD-p-nitroanilide | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |