Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.79 extracted from

  • Poe, M.; Blake, J.T.; Boulton, D.A.; Gammon, M.; Sigal, N.H.; Wu, J.K.; Zweerink, H.J.
    Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity (1991), J. Biol. Chem., 266, 98-103.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Bovine aprotinin
-
Homo sapiens
chymostatin
-
Homo sapiens
Human plasma alpha1-protease inhibitor
-
Homo sapiens
Human plasma alpha2-protease macroglobulin
-
Homo sapiens
Lima-bean trypsin inhibitor
-
Homo sapiens
phosphoramidon
-
Homo sapiens
Soybean trypsin inhibitor
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, human, SDS-PAGE, x * 45000, human, SDS-PAGE with reducing agents Homo sapiens
45000
-
x * 35000, human, SDS-PAGE, x * 45000, human, SDS-PAGE with reducing agents Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cytotoxic T-cell line Q31 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information preference for substrates with Glu or Asp as the residue amino-terminal to the scissile bond, little or no activity with oligopeptide substrates for trypsin-like, chymotrypsin-like, and elastase-like proteases Homo sapiens ?
-
?
Nalpha-tert-Butyloxycarbonyl-L-Ala-L-Ala-L-Asp thiobenzyl ester + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, human, SDS-PAGE, x * 45000, human, SDS-PAGE with reducing agents Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
Nalpha-tert-Butyloxycarbonyl-L-Ala-L-Ala-L-Asp thiobenzyl ester
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 Nalpha-tert-butyloxycarbonyl-L-Ala-L-Ala-L-Asp thiobenzyl ester Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6: about 60% of activity maximum, pH 8.5: about 45% of activity maximum Homo sapiens