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Literature summary for 3.4.21.77 extracted from

  • Waheed, A.; Hassan, M.I.; Etten, R.L.; Ahmad, F.
    Human seminal proteinase and prostate-specific antigen are the same protein (2008), J. Biosci., 33, 195-207.
    View publication on PubMed

Application

Application Comment Organism
additional information human seminal proteinase and prostate-specific antigen are identical Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM inhibits at pH 3.5 Homo sapiens
iodoacetate 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate Homo sapiens
leupeptin 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
methylmethane thiosulphonate 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
additional information 5 mM Ca2+ or p-mercaptoethanol do not inhibit Homo sapiens
N-p-tosyl-Lys chloromethyl ketone 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate Homo sapiens
N-tosyl-Phe chloromethyl ketone 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate Homo sapiens
p-hydroxyphenylmercurisulfonate 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
Phenylmethylsulphonyl fluoride 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate Homo sapiens
Zn2+ 3 mM inhibits at pH 7.5 with casein as a substrate. When bovine serum albumin is used as a substrate at pH 3.5, prostate-specific antigen or proteinase is not inhibited by 5 mM Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
calculated from the amino acid composition of isolated prostate-specific antigen and proteinase Homo sapiens
34000
-
calculated from the elution profile Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information seminal fluid Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, Tris-HCl buffer Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O is more readily hydrolysed than casein Homo sapiens ?
-
?
casein + H2O proteolytic activity at pH 7.5 Homo sapiens ?
-
?
Gelatin + H2O proteolytic activity at pH 7.5 Homo sapiens ?
-
?
N-succinyl-(Ala)3-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
?
N-succinyl-Ala-Ala-Pro-Phe-Ala-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
?
N-succinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
?
N-succinyl-Gly-Pro-Lys-p-nitroanilide + H2O prostate-specific antigen activity is 3times higher towards this substrate than towards other synthetic substrates Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
prostate-specific antigen
-
Homo sapiens
PSA
-
Homo sapiens
seminal proteinase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
bovine serum albumin is degraded 3-4times faster at pH 3.5 than at pH 7.5 Homo sapiens