Application | Comment | Organism |
---|---|---|
additional information | human seminal proteinase and prostate-specific antigen are identical | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM inhibits at pH 3.5 | Homo sapiens | |
iodoacetate | 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate | Homo sapiens | |
leupeptin | 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 | Homo sapiens | |
methylmethane thiosulphonate | 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 | Homo sapiens | |
additional information | 5 mM Ca2+ or p-mercaptoethanol do not inhibit | Homo sapiens | |
N-p-tosyl-Lys chloromethyl ketone | 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate | Homo sapiens | |
N-tosyl-Phe chloromethyl ketone | 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate | Homo sapiens | |
p-hydroxyphenylmercurisulfonate | 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 | Homo sapiens | |
Phenylmethylsulphonyl fluoride | 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate | Homo sapiens | |
Zn2+ | 3 mM inhibits at pH 7.5 with casein as a substrate. When bovine serum albumin is used as a substrate at pH 3.5, prostate-specific antigen or proteinase is not inhibited by 5 mM Zn2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
calculated from the amino acid composition of isolated prostate-specific antigen and proteinase | Homo sapiens |
34000 | - |
calculated from the elution profile | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | seminal fluid | Homo sapiens | - |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl buffer | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | is more readily hydrolysed than casein | Homo sapiens | ? | - |
? | |
casein + H2O | proteolytic activity at pH 7.5 | Homo sapiens | ? | - |
? | |
Gelatin + H2O | proteolytic activity at pH 7.5 | Homo sapiens | ? | - |
? | |
N-succinyl-(Ala)3-p-nitroanilide + H2O | prostate-specific antigen shows limited activity | Homo sapiens | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-Ala-p-nitroanilide + H2O | prostate-specific antigen shows limited activity | Homo sapiens | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O | prostate-specific antigen shows limited activity | Homo sapiens | ? | - |
? | |
N-succinyl-Gly-Pro-Lys-p-nitroanilide + H2O | prostate-specific antigen activity is 3times higher towards this substrate than towards other synthetic substrates | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
prostate-specific antigen | - |
Homo sapiens |
PSA | - |
Homo sapiens |
seminal proteinase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
bovine serum albumin is degraded 3-4times faster at pH 3.5 than at pH 7.5 | Homo sapiens |