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Literature summary for 3.4.21.77 extracted from

  • Weber, W.; Buck, F.; Meyer, A.; Hilz, H.
    Prostate specific antigen: one out of five disulfide bridges determines inactivation by reduction (2009), Biochem. Biophys. Res. Commun., 379, 1101-1106.
    View publication on PubMed
Search for more literature for 3.4.21.77

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol incubation with 3 mM for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, causes 84% loss of activity Homo sapiens
dihydrolipoate incubation with 3 mM for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, causes 65% loss of activity Homo sapiens
dithiothreitol incubation with 3 mM for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, causes a complete loss of activity. Alterates the molecular structure probably associated with an altered loading of the protein with dodecyl sulfate anions. Inactivation of the enzyme appears to follow an all-or-none reaction. Residues Cys22-Cys157 and Cys191-Cys220 are dithiothreitol-sensitive Homo sapiens
glutathione incubation with 3 mM for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, causes 35% loss of activity Homo sapiens
additional information 3 mM ascorbate for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, is devoid of inactivating potential. Loss of activity by reduction can be readily reversed by re-oxidation. Inactivation is associated with the reduction of two out of five conserved disulfides. Accessabilty of the Cys191-Cys220 disulfide near the catalytic serine 195 decides on the ability of reductants to inactivate the proteolytic activity of PSA Homo sapiens
Tris[2-carboxyethyl] phosphine incubation with 3 mM for 30 min at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8, causes 87% loss of activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07288
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
K3
-
 Homo sapiens
kallikrein 3
-
 Homo sapiens
prostate-specific antigen
-
 Homo sapiens
PSA
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.15
-
 at 25°C, in 0.1 ml 0.1 M Tris buffer, pH 7.8 Homo sapiens dithiothreitol