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Literature summary for 3.4.21.77 extracted from
- Enzymatic action of prostate-specific antigen (PSA or hK3): substrate specificity and regulation by Zn(2+), a tight-binding inhibitor (2000), Prostate, 45, 132-139.
General Stability
| General Stability | Organism |
|---|---|
| stable at 0.5 M urea in the activity assay, which is needed to prevent coagualtion of th semenogelins | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha1-Aantichymotrypsin | complex formation | Homo sapiens | |
| Cd2+ | competitive to other metal ions | Homo sapiens |  |
| Co2+ | competitive to other metal ions | Homo sapiens | |
| Cu2+ | competitive to other metal ions | Homo sapiens | |
| Hg2+ | competitive to other metal ions | Homo sapiens | |
| Zn2+ | 50% inhibition at 0.02 mM, 25fold molar excess of Zn2+ to enzyme; competitive to substrate and other metal ions, modeling; strong, tight-binding inhibitor | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polypeptide + H2O | Homo sapiens | - |
peptides | - |
? | |
| semenogelin + H2O | Homo sapiens | semenogelins I and II, cleavage results in liquefaction and release of motil spermatozoa | semenogelin fractions | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Preferential cleavage: -Tyr-/- | cleaves at the C-terminal side of several amino acids residues, like tyrosine, glutamine, histidine, asparic acid, leucine, serine, and asparagine, not at arginine, lysine, phenylalanine, tryptophan, or methionine residues | Homo sapiens | |
| Preferential cleavage: -Tyr-/- | enzyme shows a substrate specificity different from trypsin, chymotrypsin, and kallikrein 1 and 2 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| semen | - |
Homo sapiens | - |
| seminal plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O | chromogenic substrate | Homo sapiens | methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline | - |
? | |
| methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O | i.e. S2586 | Homo sapiens | methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline | - |
? | |
| polypeptide + H2O | - |
Homo sapiens | peptides | - |
? | |
| semenogelin + H2O | semenogelins I and II | Homo sapiens | semenogelin fragments | - |
? | |
| semenogelin + H2O | semenogelin I contains 18 cleavage sites, semenogelin II contains 16 cleavage sites | Homo sapiens | semenogelin fragments | - |
? | |
| semenogelin + H2O | semenogelins I and II, cleavage results in liquefaction and release of motil spermatozoa | Homo sapiens | semenogelin fractions | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| K3 | - |
Homo sapiens |
| prostate-specific antigen | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0056 | - |
Zn2+ | pH 8.0, 37°C, with methoxy-succinyl-Arg-Pro-Tyr-4-nitrophenyl | Homo sapiens | |
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Release 2023.1 (January 2023)
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