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Literature summary for 3.4.21.75 extracted from

  • Dragulescu-Andrasi, A.; Liang, G.; Rao, J.
    In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates (2009), Bioconjug. Chem., 20, 1660-1666.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.75

Application

Application Comment Organism
analysis bioluminogenic probes can specifically image furin activity in xenografted breast cancer tumors in mice Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Mus musculus
Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the bioluminescence emission in the presence of firefly luciferase is furin-dependent and specific in living mice, overview ?
-
 ?
additional information Homo sapiens the bioluminescence emission in the presence of firefly luciferase, recombinantly expressed as GFP-tagged enzyme in human MDA-MB-468 cells, breast adenocarcinoma cells, is furin-dependent and specific ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-
Mus musculus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Mus musculus
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breast cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-RVRR-4-methylcoumarin 7-amide + H2O
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 Mus musculus acetyl-RVRR + 7-amino-4-methylcoumarin
-
 ?
acetyl-RVRR-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens acetyl-RVRR + 7-amino-4-methylcoumarin
-
 ?
acetyl-RVRR-aminoluciferin + H2O the substrate consists of D-aminoluciferin coupled to the C-terminus of furin recognition peptide sequence, furin cleaves at the C-terminal to the last arginine residue. In the presence of furin, the probes are hydrolyzed to remove the peptide caging group and generate free D-aminoluciferin which subsequently produces light emission in the presence of firefly luciferase Mus musculus acetyl-RVRR + D-aminoluciferin
-
 ?
acetyl-RVRR-aminoluciferin + H2O the substrate consists of D-aminoluciferin coupled to the C-terminus of furin recognition peptide sequence, furin cleaves at the C-terminal to the last arginine residue. In the presence of furin, the probes are hydrolyzed to remove the peptide caging group and generate free D-aminoluciferin which subsequently produces light emission in the presence of firefly luciferase Homo sapiens acetyl-RVRR + D-aminoluciferin
-
 ?
acetyl-RYKR-4-methylcoumarin 7-amide + H2O
-
 Mus musculus acetyl-RYKR + 7-amino-4-methylcoumarin
-
 ?
acetyl-RYKR-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens acetyl-RYKR + 7-amino-4-methylcoumarin
-
 ?
acetyl-RYKR-aminoluciferin + H2O the substrate consists of D-aminoluciferin coupled to the C-terminus of furin recognition peptide sequence, furin cleaves at the C-terminal to the last arginine residue. In the presence of furin, the probes are hydrolyzed to remove the peptide caging group and generate free D-aminoluciferin which subsequently produces light emission in the presence of firefly luciferase Mus musculus acetyl-RYKR + D-aminoluciferin
-
 ?
acetyl-RYKR-aminoluciferin + H2O the substrate consists of D-aminoluciferin coupled to the C-terminus of furin recognition peptide sequence, furin cleaves at the C-terminal to the last arginine residue. In the presence of furin, the probes are hydrolyzed to remove the peptide caging group and generate free D-aminoluciferin which subsequently produces light emission in the presence of firefly luciferase Homo sapiens acetyl-RYKR + D-aminoluciferin
-
 ?
additional information the bioluminescence emission in the presence of firefly luciferase is furin-dependent and specific in living mice, overview Mus musculus ?
-
 ?
additional information the bioluminescence emission in the presence of firefly luciferase, recombinantly expressed as GFP-tagged enzyme in human MDA-MB-468 cells, breast adenocarcinoma cells, is furin-dependent and specific Homo sapiens ?
-
 ?
additional information furin performs a calcium-dependent proteolytic cleavage at the C-terminus of a consensus amino acid motif R-X-K/R-R, with X being any amino acid. This tetrapeptide motif provides sufficient specificity to bind the active furin Mus musculus ?
-
 ?
additional information furin performs a calcium-dependent proteolytic cleavage at the C-terminus of a consensus amino acid motif R-X-K/R-R, with X being any amino acid. This tetrapeptide motif provides sufficient specificity to bind the active furin Homo sapiens ?
-
 ?

General Information

General Information Comment Organism
physiological function the bioluminescence emission in the presence of firefly luciferase is furin-dependent and specific, furin activates the luciferase activity 30fold Mus musculus
physiological function the bioluminescence emission in the presence of firefly luciferase is furin-dependent and specific, furin activates the luciferase activity 7fold Homo sapiens