Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | parasite expresses a Ca2+ dependent, furin-like protease activity | Cryptosporidium parvum |
Application | Comment | Organism |
---|---|---|
medicine | furin-like protease activity may be involved in mediating host-parasite interactions. Inhibition of infection is due to an effect on the parasite-derived furin activity rather than the host cell furin | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Cryptosporidium parvum | |
4-(2-aminoethyl)benzenesulphonyl fluoride | - |
Cryptosporidium parvum | |
decanoyl-Arg-Val-Lys-Arg-chloromethylketone | most inhibitory, inhibits Cryptosporidium parvum infection of HCT-8 cells in a dose-dependent manner, with ca. 50% inhibition occurring at a concentration of 0.01 mM | Cryptosporidium parvum | |
EDTA | 97% inhibition, Ca2+ can reverse the inhibitory effect | Cryptosporidium parvum | |
EDTA | completely inhibits | Homo sapiens | |
EGTA | - |
Cryptosporidium parvum | |
Glu-Gly-Arg-chloromethylketone | poor inhibitor | Cryptosporidium parvum | |
leupeptin | - |
Cryptosporidium parvum | |
additional information | aprotinin has no effect on furin-like protease activity | Cryptosporidium parvum | |
N-tosyl-L-lysine chloromethyl ketone | - |
Cryptosporidium parvum | |
Phe-Pro-Arg-chloromethylketone | poor inhibitor | Cryptosporidium parvum | |
phenylmethylsulfonyl fluoride | - |
Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01517 | - |
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin | - |
Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | - |
- |
- |
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-8 cell | - |
Homo sapiens | - |
additional information | lysate | Cryptosporidium parvum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gp40/15 + H2O | cleaves recombinant Cryptosporidium parvum and Cryptosporidium hominis gp40/15. Putative furin cleavage site RSRR | Homo sapiens | gp40 + gp15 | - |
? | |
gp40/15 + H2O | putative furin cleavage site RSRR | Cryptosporidium parvum | gp40 + gp15 | - |
? | |
gp40/15 subtype 1e + H2O | RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 | Homo sapiens | gp40 + gp15 | - |
? | |
gp40/15 subtype 1e + H2O | RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 | Cryptosporidium parvum | gp40 + gp15 | - |
? | |
additional information | mutation in the RSRR cleavage site prevents processing of gp40/15 | Homo sapiens | ? | - |
? | |
additional information | mutation in the RSRR cleavage site prevents processing of gp40/15 | Cryptosporidium parvum | ? | - |
? | |
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Cryptosporidium parvum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
furin-like protease | - |
Cryptosporidium parvum |
subtilisin-like proprotein convertase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cryptosporidium parvum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Cryptosporidium parvum |