Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.75 extracted from

  • Zhou, Y.; Lindberg, I.
    Purification and characterization of the prohormone convertase PC1(PC3) (1993), J. Biol. Chem., 268, 5615-5623.
    View publication on PubMed
Search for more literature for 3.4.21.75

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
D-Tyr-Ala-Lys-Arg-CH2Cl
-
 Mus musculus
EDTA
-
 Mus musculus
p-chloromercuribenzenesulfonic acid
-
 Mus musculus
Pro-Gly-Lys-Arg-CH2Cl
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
Ca2+ 5 mM Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus role in processing of proenkephalin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 expression in Chinese hamster ovary cells
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-10°C, 10% glycerol, 90% loss of activity after 1 week Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Carboxybenzyl-Arg-Ser-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
additional information synthetic tripeptide substrates containing only pairs of basic amino acids are not well cleaved Mus musculus ?
-
 ?
additional information the term -+- depicts the point of cleavage, the enzyme prefers substrates with an arginine 4-amino acid amino-terminal to the cleavage site Mus musculus ?
-
 ?
additional information role in processing of proenkephalin Mus musculus ?
-
 ?
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus ?
-
 ?
tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
 Mus musculus