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Literature summary for 3.4.21.75 extracted from

  • Hatsuzawa, K.; Nagahama, M.; Takahashi, S.; Takada, K.; Murakami, K.; Nakayama, K.
    Purification and characterization of furin, a Kex2-like processing endoprotease, produced in Chinese hamster ovary cells (1992), J. Biol. Chem., 267, 16094-16099.
    View publication on PubMed
Search for more literature for 3.4.21.75

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Mus musculus
Cu2+
-
 Mus musculus
diisopropyl fluorophosphate moderately Mus musculus
EDTA
-
 Mus musculus
Hg2+
-
 Mus musculus
p-chloromercuribenzenesulfonic acid
-
 Mus musculus
phenylmethanesulfonyl fluoride moderately Mus musculus
Zn2+
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Mus musculus 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
CoCl2 partially restores activity after EDTA treatment Mus musculus
MnCl2 partially restores activity after EDTA treatment Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 3 different forms, MW 81000, 83000 and 96000, may be produced by differential processing of a furin molecule and mature furin may be autocatalytically produced Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 expression in Chinese hamster ovary cells
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Protein precursor + H2O cleavage of-Arg-Xaa-Yaa-Arg-+- bonds where Xaa can be any amino acid and Yaa is Arg or Lys Mus musculus ?
-
 ?
tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Mus musculus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mus musculus