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Literature summary for 3.4.21.74 extracted from
- The coagulant enzyme from Bothrops atrox venom (batroxobin) (1976), Methods Enzymol., 45, 214-223.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| imidazole | activation | Bothrops atrox |  |
| imidazole | activation | Bothrops asper | |
| imidazole | activation | Bothrops moojeni | |
| imidazole | activation | Bothrops marajoensis | |
| additional information | no activation by phenol | Bothrops atrox | |
| additional information | no activation by phenol | Bothrops asper | |
| additional information | no activation by phenol | Bothrops moojeni | |
| additional information | no activation by phenol | Bothrops marajoensis |
General Stability
| General Stability | Organism |
|---|---|
| Stable to repeated freeze-thawing | Bothrops moojeni |
| Stable to repeated freeze-thawing | Bothrops marajoensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops asper | |
| diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops atrox | |
| diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops marajoensis | |
| diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops moojeni | |
| additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops asper | |
| additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops atrox | |
| additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops marajoensis | |
| additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops moojeni | |
| Soybean trypsin inhibitor | not | Bothrops asper | |
| Soybean trypsin inhibitor | not | Bothrops atrox | |
| Soybean trypsin inhibitor | not | Bothrops marajoensis | |
| Soybean trypsin inhibitor | not | Bothrops moojeni |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bothrops atrox | - |
- |
| soluble | - |
Bothrops asper | - |
- |
| soluble | - |
Bothrops moojeni | - |
- |
| soluble | - |
Bothrops marajoensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops asper |
| 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops moojeni |
| 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops marajoensis |
| 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops asper |
| 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops moojeni |
| 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops marajoensis |
| 37000 | - |
Bothrops moojeni, ultracentrifugation | Bothrops moojeni |
| 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops asper |
| 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops moojeni |
| 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops marajoensis |
| 43000 | - |
Bothrops marajoensis, ultracentrifugation | Bothrops marajoensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bothrops asper | - |
Bothrops atrox variety | - |
| Bothrops atrox | - |
South American pit viper, formerly EC 3.4.21.29, different varieties | - |
| Bothrops marajoensis | - |
Bothrops atrox variety | - |
| Bothrops moojeni | - |
Bothrops atrox variety | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | single-chain glycopeptide | Bothrops asper |
| glycoprotein | single-chain glycopeptide | Bothrops moojeni |
| glycoprotein | single-chain glycopeptide | Bothrops marajoensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Bothrops atrox variety | Bothrops asper |
| Bothrops atrox variety | Bothrops moojeni |
| Bothrops atrox variety | Bothrops marajoensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Bothrops atrox | - |
| venom | - |
Bothrops asper | - |
| venom | - |
Bothrops moojeni | - |
| venom | - |
Bothrops marajoensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bothrops atrox |
| additional information | - |
- |
Bothrops asper |
| additional information | - |
- |
Bothrops moojeni |
| additional information | - |
- |
Bothrops marajoensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in dilute solution in physiological saline, pH 6, containing 0.02% gelatin and 0.3% of chlorobutol, enzyme isolated from Bothrops moojeni and Bothrops marajoensis, more than a year | Bothrops moojeni |
| 4°C, in dilute solution in physiological saline, pH 6, containing 0.02% gelatin and 0.3% of chlorobutol, enzyme isolated from Bothrops moojeni and Bothrops marajoensis, more than a year | Bothrops marajoensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops atrox | ? | - |
? | |
| Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops asper | ? | - |
? | |
| Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops moojeni | ? | - |
? | |
| Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops marajoensis | ? | - |
? | |
| Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops atrox | ? | - |
? | |
| Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops asper | ? | - |
? | |
| Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops moojeni | ? | - |
? | |
| Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops marajoensis | ? | - |
? | |
| Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops atrox | ? | - |
? | |
| Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops asper | ? | - |
? | |
| Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops moojeni | ? | - |
? | |
| Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops marajoensis | ? | - |
? | |
| Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops atrox | ? | - |
? | |
| Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops asper | ? | - |
? | |
| Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops moojeni | ? | - |
? | |
| Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops marajoensis | ? | - |
? | |
| Human blood coagulation factor XIII + H2O | activation, Bothrops asper and Bothrops moojeni, but not Bothrops majaroensis enzyme | Bothrops asper | Activated human blood coagulation factor XIII | - |
? | |
| Human blood coagulation factor XIII + H2O | activation, Bothrops asper and Bothrops moojeni, but not Bothrops majaroensis enzyme | Bothrops moojeni | Activated human blood coagulation factor XIII | - |
? | |
| additional information | no activation of human factor VIII | Bothrops marajoensis | ? | - |
? | |
| additional information | no activation of plasminogen | Bothrops atrox | ? | - |
? | |
| additional information | no activation of plasminogen | Bothrops asper | ? | - |
? | |
| additional information | no activation of plasminogen | Bothrops moojeni | ? | - |
? | |
| additional information | no activation of plasminogen | Bothrops marajoensis | ? | - |
? | |
| additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops atrox | ? | - |
? | |
| additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops asper | ? | - |
? | |
| additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops moojeni | ? | - |
? | |
| additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops marajoensis | ? | - |
? | |
| additional information | fibrinogen beta-chain | Bothrops atrox | ? | - |
? | |
| additional information | fibrinogen beta-chain | Bothrops asper | ? | - |
? | |
| additional information | fibrinogen beta-chain | Bothrops moojeni | ? | - |
? | |
| additional information | fibrinogen beta-chain | Bothrops marajoensis | ? | - |
? | |
| Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops atrox | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops asper | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops moojeni | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops marajoensis | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| Tosyl-L-arginine methyl ester + H2O | - |
Bothrops atrox | Tosyl-L-arginine + methanol | - |
? | |
| Tosyl-L-arginine methyl ester + H2O | - |
Bothrops asper | Tosyl-L-arginine + methanol | - |
? | |
| Tosyl-L-arginine methyl ester + H2O | - |
Bothrops moojeni | Tosyl-L-arginine + methanol | - |
? | |
| Tosyl-L-arginine methyl ester + H2O | - |
Bothrops marajoensis | Tosyl-L-arginine + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops asper |
| monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops moojeni |
| monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops marajoensis |
| monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops asper |
| monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops moojeni |
| monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops marajoensis |
| monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops asper |
| monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops moojeni |
| monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops marajoensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Batroxobin | - |
Bothrops atrox |
| Batroxobin | - |
Bothrops asper |
| Batroxobin | - |
Bothrops moojeni |
| Batroxobin | - |
Bothrops marajoensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bothrops atrox |
| 37 | - |
assay at | Bothrops asper |
| 37 | - |
assay at | Bothrops moojeni |
| 37 | - |
assay at | Bothrops marajoensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
1 h, in glycerol, stable | Bothrops moojeni |
| 100 | - |
1 h, in glycerol, stable | Bothrops marajoensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops atrox |
| additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops asper |
| additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops moojeni |
| additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops marajoensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 9 | several h stable at 20°C | Bothrops moojeni |
| 2.5 | 9 | several h stable at 20°C | Bothrops marajoensis |
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