Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the N-terminal growth factor domain of uPA reduced the affinity for enzyme receptors 2-4fold, depending on the receptor, e.g. VLDLR-I, SorLA, or LRP-1A, and deletion of both the growth factor domain and the kringle reduced the receptor affinity 7fold, overview | Homo sapiens |
R178A/R179A/R181A | site-directed mutagenesis, the mutant shows reduced receptor binding | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
plasminogen activator inhibitor-1 | i.e. PAI-1, complexes the enzyme, binding structure, the 10-100-fold higher affinity of the uPA-PAI-1 complex compared with the free components depends on the bonus effect of bringing the binding areas on uPA and PAI-1 together on the same binding entity, overview, inhibitor mutants K71A/R78A/Y81A/K82A, K82A-R120A, and R78A-K124A show reduced binding to the enzyme and the enzyme receptors, effects of several mutations of the inhibitor protein on complex formation and endocytosis, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of enzyme with enzyme receptors VLDLR-I, SorLA, or LRP-1A , overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme-plasminogen activator inhibitor-1 complex in intenalized by endocytosis via binding of membrane-bound receptors, overview | ? | - |
? | |
plasminogen + H2O | Homo sapiens | - |
plasmin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme-plasminogen activator inhibitor-1 complex in intenalized by endocytosis via binding of membrane-bound receptors, overview | Homo sapiens | ? | - |
? | |
plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
S-2444 + H2O | i.e. pyro-Glu-Gly-Arg-p-nitroanilide, a chromogenic substrate | Homo sapiens | pyroGlu-Gly-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
uPA | - |
Homo sapiens |
Urokinase-type plasminogen activator | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |