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Literature summary for 3.4.21.73 extracted from

  • Sun, Z.; Liu, J.N.
    Mutagenesis at Pro309 of single-chain urokinase-type plasminogen activator alters its catalytic properties (2005), Proteins, 61, 870-877.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
P309A 60% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309D 200% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin Homo sapiens
P309F 500% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin Homo sapiens
P309G 100% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309H 400% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin Homo sapiens
P309L 250% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309N 150% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309R 350% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin Homo sapiens
P309S 50% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309T 60% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin Homo sapiens
P309V 80% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens
P309W 700% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide wild-type, pH 8.0, 22°C Homo sapiens
0.079
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309S, pH 8.0, 22°C Homo sapiens
0.081
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309A, pH 8.0, 22°C Homo sapiens
0.087
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309AT, pH 8.0, 22°C Homo sapiens
0.102
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309V, pH 8.0, 22°C Homo sapiens
0.11
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309G, pH 8.0, 22°C Homo sapiens
0.129
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309N, pH 8.0, 22°C Homo sapiens
0.153
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309L, pH 8.0, 22°C Homo sapiens
0.175
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309D, pH 8.0, 22°C Homo sapiens
0.235
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309R, pH 8.0, 22°C Homo sapiens
0.268
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309H, pH 8.0, 22°C Homo sapiens
0.361
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309F, pH 8.0, 22°C Homo sapiens
0.452
-
L-pyroGlu-Gly-L-Arg-4-nitroanilide mutant P309W, pH 8.0, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-pyroGlu-Gly-L-Arg-4-nitroanilide + H2O i.e. S-2444 Homo sapiens L-pyroGlu-Gly-L-Arg + 4-nitroaniline
-
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