Protein Variants | Comment | Organism |
---|---|---|
P309A | 60% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309D | 200% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin | Homo sapiens |
P309F | 500% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin | Homo sapiens |
P309G | 100% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309H | 400% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin | Homo sapiens |
P309L | 250% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309N | 150% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309R | 350% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin | Homo sapiens |
P309S | 50% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309T | 60% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide, about 15% decrease in activation by Lys-plasmin | Homo sapiens |
P309V | 80% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
P309W | 700% increase in KM-value for L-pyroGlu-Gly-L-Arg-4-nitroanilide | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | wild-type, pH 8.0, 22°C | Homo sapiens | |
0.079 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309S, pH 8.0, 22°C | Homo sapiens | |
0.081 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309A, pH 8.0, 22°C | Homo sapiens | |
0.087 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309AT, pH 8.0, 22°C | Homo sapiens | |
0.102 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309V, pH 8.0, 22°C | Homo sapiens | |
0.11 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309G, pH 8.0, 22°C | Homo sapiens | |
0.129 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309N, pH 8.0, 22°C | Homo sapiens | |
0.153 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309L, pH 8.0, 22°C | Homo sapiens | |
0.175 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309D, pH 8.0, 22°C | Homo sapiens | |
0.235 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309R, pH 8.0, 22°C | Homo sapiens | |
0.268 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309H, pH 8.0, 22°C | Homo sapiens | |
0.361 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309F, pH 8.0, 22°C | Homo sapiens | |
0.452 | - |
L-pyroGlu-Gly-L-Arg-4-nitroanilide | mutant P309W, pH 8.0, 22°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-pyroGlu-Gly-L-Arg-4-nitroanilide + H2O | i.e. S-2444 | Homo sapiens | L-pyroGlu-Gly-L-Arg + 4-nitroaniline | - |
? |