Cloned (Comment) | Organism |
---|---|
cDNA cloning | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E301A | site-directed mutagenesis | Homo sapiens |
E301D | site-directed mutagenesis | Homo sapiens |
E301H | site-directed mutagenesis | Homo sapiens |
K300A | site-directed mutagenesis | Homo sapiens |
K300H | site-directed mutagenesis | Homo sapiens |
K300W | site-directed mutagenesis | Homo sapiens |
K313A | site-directed mutagenesis | Homo sapiens |
S303E | site-directed mutagenesis | Homo sapiens |
Y306G | site-directed mutagenesis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
S2444 | pH 7.4, 25°C, wild-type and mutant E301A, single-chain activator | Homo sapiens | |
0.052 | - |
S2444 | pH 7.4, 25°C, mutant K300H, single-chain activator | Homo sapiens | |
0.053 | - |
S2444 | pH 7.4, 25°C, mutant S303E, single-chain activator | Homo sapiens | |
0.054 | - |
S2444 | pH 7.4, 25°C, mutant K300A and K313A, single-chain activator | Homo sapiens | |
0.055 | - |
S2444 | pH 7.4, 25°C, mutant E301H, single-chain activator | Homo sapiens | |
0.058 | - |
S2444 | pH 7.4, 25°C, mutant Y306G, single-chain activator | Homo sapiens | |
0.059 | - |
S2444 | pH 7.4, 25°C, mutant K300W, single-chain activator | Homo sapiens | |
0.06 | - |
S2444 | pH 7.4, 25°C, mutant E301D, single-chain activator | Homo sapiens | |
0.074 | - |
S2444 | pH 7.4, 25°C, mutant K300W, two-chain activator | Homo sapiens | |
0.075 | - |
S2444 | pH 7.4, 25°C, mutant K300H, two-chain activator | Homo sapiens | |
0.077 | - |
S2444 | pH 7.4, 25°C, mutant K300A and S303E, two-chain activator | Homo sapiens | |
0.078 | - |
S2444 | pH 7.4, 25°C, wild-type, two-chain activator | Homo sapiens | |
0.08 | - |
S2444 | pH 7.4, 25°C, mutant K313A, two-chain activator | Homo sapiens | |
0.082 | - |
S2444 | pH 7.4, 25°C, mutant E301D, two-chain activator | Homo sapiens | |
0.085 | - |
S2444 | pH 7.4, 25°C, mutant Y306G, two-chain activator | Homo sapiens | |
0.087 | - |
S2444 | pH 7.4, 25°C, mutant E301A, two-chain activator | Homo sapiens | |
0.089 | - |
S2444 | pH 7.4, 25°C, mutant E301H, two-chain activator | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | Homo sapiens | principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing | plasmin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | urokinase-type plasminogen activator includes its single-chain zymogen, pro-urokinase and two-chain enzyme urokinase | Homo sapiens | ? | - |
? | |
plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
plasminogen + H2O | principal function is fibrinolysis, u-PA also been implicated in other physiological functions such as embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing | Homo sapiens | plasmin + ? | - |
? | |
S2444 + H2O | i.e. 5-oxo-L-Pro-Gly-L-Arg-p-nitroanilide | Homo sapiens | pyroGlu-Gly-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UK | - |
Homo sapiens |
Urokinase-type plasminogen activator | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
S2444 | pH 7.4, 25°C, mutant K300A, single-chain activator | Homo sapiens | |
0.0085 | - |
S2444 | pH 7.4, 25°C, mutant K300W, single-chain activator | Homo sapiens | |
0.027 | - |
S2444 | pH 7.4, 25°C, mutant E301A, single-chain activator | Homo sapiens | |
0.029 | - |
S2444 | pH 7.4, 25°C, mutant Y306G, single-chain activator | Homo sapiens | |
0.047 | - |
S2444 | pH 7.4, 25°C, mutant E301D, single-chain activator | Homo sapiens | |
0.065 | - |
S2444 | pH 7.4, 25°C, mutant K300H, single-chain activator | Homo sapiens | |
0.32 | - |
S2444 | pH 7.4, 25°C, mutant S303E, single-chain activator | Homo sapiens | |
0.32 | - |
S2444 | pH 7.4, 25°C, wild-type, single-chain activator | Homo sapiens | |
0.33 | - |
S2444 | pH 7.4, 25°C, mutant K313A, single-chain activator | Homo sapiens | |
1.5 | - |
S2444 | pH 7.4, 25°C, mutant E301H, single-chain activator | Homo sapiens | |
22 | - |
S2444 | pH 7.4, 25°C, mutant Y306G, two-chain activator | Homo sapiens | |
27 | - |
S2444 | pH 7.4, 25°C, mutant K300W, two-chain activator | Homo sapiens | |
60 | - |
S2444 | pH 7.4, 25°C, mutant K300A, two-chain activator | Homo sapiens | |
83 | - |
S2444 | pH 7.4, 25°C, mutant E301H, two-chain activator | Homo sapiens | |
180 | - |
S2444 | pH 7.4, 25°C, wild-type and mutant S303E, two-chain activator | Homo sapiens | |
190 | - |
S2444 | pH 7.4, 25°C, mutant K313A, two-chain activator | Homo sapiens | |
200 | - |
S2444 | pH 7.4, 25°C, mutant E301A, two-chain activator | Homo sapiens | |
270 | - |
S2444 | pH 7.4, 25°C, mutant E301D, two-chain activator | Homo sapiens | |
350 | - |
S2444 | pH 7.4, 25°C, mutant K300H, two-chain activator | Homo sapiens |