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Literature summary for 3.4.21.70 extracted from

  • Mallory, P.A.; Travis, J.
    Human pancreatic enzymes: purification and characterization of a nonelastolytic enzyme, protease E. resembling elastase (1975), Biochemistry, 14, 722-730.
    View publication on PubMed
Search for more literature for 3.4.21.70

General Stability

General Stability Organism
Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
 Homo sapiens
Acetyl-Ala-Ala-AlaCH2Cl
-
 Homo sapiens
Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
 Homo sapiens
Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
 Homo sapiens
Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
 Homo sapiens
Human serum alpha1-antitrypsin no inhibition by other naturally occuring inhibitors tested Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.166
-
 tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 human, gel filtration Homo sapiens
30500
-
 1 * 30500, human, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
 Homo sapiens Carboxybenzoyl-L-alanine + 4-nitrophenol
-
 ?
casein + H2O
-
 Homo sapiens hydrolyzed casein
-
 ?
Hemoglobin + H2O
-
 Homo sapiens Hydrolyzed hemoglobin
-
 ?
additional information no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester Homo sapiens ?
-
 ?
additional information no elastolytic activity Homo sapiens ?
-
 ?
Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
 Homo sapiens Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30500, human, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 9.5 casein Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0 Homo sapiens