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Literature summary for 3.4.21.7 extracted from

  • Aaltonen, T.; Ollikainen, P.
    Effect of microfiltration of milk on plasmin activity (2011), Int. Dairy J., 21, 193-197.
    View publication on PubMed
Search for more literature for 3.4.21.7

Activating Compound

Activating Compound Comment Organism Structure
additional information the increased activity of plasmin after diafiltration may be also due to elimination of small enzyme inhibitor proteins Bos taurus

Application

Application Comment Organism
food industry milk retentate with increased plasmin activity is an interesting starting material for cheese-making. Increased plasmin activity increases cheese flavour and decreases ripening time Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
alpha-lactalbumin shows a strong correlation with plasmin activity and may have inhibitory activity against plasmin Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme activity follows Michaelis-Menten kinetics Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bos taurus
-
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Val-L-Leu-L-Lys-4-nitroanilide + H2O
-
 Bos taurus D-Val-L-Leu-L-Lys + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
fibrinase
-
 Bos taurus
fibrinolysin
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the increased activity of plasmin after heat treatment may be also due to inactivation of enzyme inhibitor proteins Bos taurus
95
-
 pasteurization of whey protein-free retentate of micro- and difiltrated milk at 95°C for 15 s does not significantly affect plasmin or plasminogen-derived activities. The retentate contains increased plasmin activity, proportional to the concentration of beta-lactoglobulin Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

General Information

General Information Comment Organism
additional information pasteurization of whey protein-free retentate of micro- and difiltrated milk at 95°C for 15 s does not significantly affect plasmin or plasminogen-derived activities. The retentate contains increased plasmin activity, proportional to the concentration of beta-lactoglobulin Bos taurus