Application | Comment | Organism |
---|---|---|
medicine | leukocyte integrins are cellular receptors for recombinant activated protein C and the interaction decreases neutrophil recruitment into tissues, providing a potential mechanism by which recombinant human APC may protect against sepsis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant cloned into pcDNA 3.1(+)/Hygro vector and expressed in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D222E | mutation of the Arg-Gly-Asp sequence abolishes both integrin binding and inhibition of neutrophil migration | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of blocking mAbs against beta1 or beta3 integrins partially displaces recombinant activated protein C from the cell surface. Cyclic RGD peptide Ac-c[(Pen)-Tyr(Me)-Ala-Arg-Gly-Asp-Asn-Tic-Cys]NH2 also significantly inhibits recombinant activated protein C binding, whereas blocking anti-beta2 integrin (non-RGD binding integrin) mAb has no effect | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | significantly increases recombinant activated protein C binding to the neutrophil surface | Homo sapiens | |
Mg2+ | significantly increases recombinant activated protein C binding to the neutrophil surface | Homo sapiens | |
Mn2+ | significantly increases recombinant activated protein C binding to the neutrophil surface | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | directly binds to beta1 and beta3 integrins and inhibits neutrophil migration, both in vitro and in vivo. Activated protein C possesses an Arg-Gly-Asp sequence, which is critical for the inhibition. The enzyme does not functionally alter the signaling pathway associated with chemotaxis receptors on neutrophil | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Activated protein C | - |
Homo sapiens |