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Literature summary for 3.4.21.69 extracted from

  • Elphick, G.F.; Sarangi, P.P.; Hyun, Y.M.; Hollenbaugh, J.A.; Ayala, A.; Biffl, W.L.; Chung, H.L.; Rezaie, A.R.; McGrath, J.L.; Topham, D.J.; Reichner, J.S.; Kim, M.
    Recombinant human activated protein C inhibits integrin-mediated neutrophil migration (2009), Blood, 113, 4078-4085.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine leukocyte integrins are cellular receptors for recombinant activated protein C and the interaction decreases neutrophil recruitment into tissues, providing a potential mechanism by which recombinant human APC may protect against sepsis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
mutant cloned into pcDNA 3.1(+)/Hygro vector and expressed in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D222E mutation of the Arg-Gly-Asp sequence abolishes both integrin binding and inhibition of neutrophil migration Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information addition of blocking mAbs against beta1 or beta3 integrins partially displaces recombinant activated protein C from the cell surface. Cyclic RGD peptide Ac-c[(Pen)-Tyr(Me)-Ala-Arg-Gly-Asp-Asn-Tic-Cys]NH2 also significantly inhibits recombinant activated protein C binding, whereas blocking anti-beta2 integrin (non-RGD binding integrin) mAb has no effect Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ significantly increases recombinant activated protein C binding to the neutrophil surface Homo sapiens
Mg2+ significantly increases recombinant activated protein C binding to the neutrophil surface Homo sapiens
Mn2+ significantly increases recombinant activated protein C binding to the neutrophil surface Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information directly binds to beta1 and beta3 integrins and inhibits neutrophil migration, both in vitro and in vivo. Activated protein C possesses an Arg-Gly-Asp sequence, which is critical for the inhibition. The enzyme does not functionally alter the signaling pathway associated with chemotaxis receptors on neutrophil Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Activated protein C
-
Homo sapiens