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Literature summary for 3.4.21.69 extracted from

  • Friedrich, U.; Blom, A.M.; Dahlbaeck, B.; Villoutreix, B.O.
    Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C (2001), J. Biol. Chem., 276, 24122-24128.
    View publication on PubMed
Search for more literature for 3.4.21.69

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
K37S/K38Q/K39Q created by site-directed in vitro mutagenesis Homo sapiens
K37S/K38Q/K39Q/K62N/K63D created by site-directed in vitro mutagenesis Homo sapiens
R74Q created by site-directed in vitro mutagenesis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
protein C inhibitor PCI inhibition stimulated by heparin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 S-2366 pH 7.4, 37°C, mutant R74Q Homo sapiens
0.00091
-
 S-2366 pH 7.4, 37°C, wild type Homo sapiens
0.00093
-
 S-2366 pH 7.4, 37°C, mutant K37S/K38Q/K39Q/K62N/K63D Homo sapiens
0.00095
-
 S-2366 pH 7.4, 37°C, mutant K62N/K63D Homo sapiens
0.00098
-
 S-2366 pH 7.4, 37°C, mutant K37S/K38Q/K39Q Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Factor Va + H2O Homo sapiens natural anticoagulant, inhibits thrombin generation by degrading factors Va and VIIIa ?
-
 ?
Factor VIIIa + H2O Homo sapiens natural anticoagulant, inhibits thrombin generation by degrading factors Va and VIIIa ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor Va + H2O natural anticoagulant, inhibits thrombin generation by degrading factors Va and VIIIa Homo sapiens ?
-
 ?
Factor VIIIa + H2O natural anticoagulant, inhibits thrombin generation by degrading factors Va and VIIIa Homo sapiens ?
-
 ?
S-2366 + H2O i.e. 5-oxo-L-Pro-L-Pro-L-Arg-p-anitroanilide Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
70
-
 S-2366 pH 7.4, 37°C, wild type Homo sapiens
71
-
 S-2366 pH 7.4, 37°C, mutant K37S/K38Q/K39Q/K62N/K63D Homo sapiens
75
-
 S-2366 pH 7.4, 37°C, mutant K37S/K38Q/K39Q Homo sapiens
76
-
 S-2366 pH 7.4, 37°C, mutant R74Q Homo sapiens
78
-
 S-2366 pH 7.4, 37°C, mutant K62N/K63D Homo sapiens