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Literature summary for 3.4.21.64 extracted from
- Proteinase K activity determination with beta-galactosidase as sensitive macromolecular substrate (2016), Anal. Biochem., 513, 54-60 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme proK. Compared to free proK, immobilized proK is much less efficient in inactivating beta-galactosidase, most likely due to a decreased mobility of immobilized proK and a restricted accessibility of the substrate to the active site of proK | Parengyodontium album |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PMSF | irreversible, covalent proK inhibitor | Parengyodontium album |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics, overview | Parengyodontium album |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parengyodontium album | P06873 | i.e. Tritirachium album or Engyodontium album | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | recombinant enzyme expressed from Pichia pastoris | Parengyodontium album | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-galactosidase + H2O | proteinase K activity determination with beta-galactosidase as sensitive macromolecular substrate, comparison of the native protein-attacking ability of free and immobilized proK, method evaluation, overview. beta-Galactosidase is inactivated by proK. Compared to free proK, immobilized proK is much less efficient in inactivating beta-galactosidase, most likely due to a decreased mobility of immobilized proK and a restricted accessibility of the substrate to the active site of proK | Parengyodontium album | ? | - |
? | |
| additional information | beta-galactosidase activity is measured spectrophotometrically with 2-nitrophenyl-beta-galactopyranoside. Hen egg lysozyme, horseradish peroxidase, or Aspergillus sp. glucose oxidase are not inactivated by proteinase K | Parengyodontium album | ? | - |
? | |
| succinyl-AAPF-4-nitroanilide + H2O | - |
Parengyodontium album | succinyl-AAPF + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PROK | - |
Parengyodontium album |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
recombinant enzyme | Parengyodontium album |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
recombinant enzyme | Parengyodontium album |
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Release 2023.1 (January 2023)
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