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Literature summary for 3.4.21.64 extracted from
- Effect of alkyl alcohols on partially unfolded state of proteinase K: Differential stability of alpha-helix and beta-sheet rich regions of the enzyme (2009), Biochimie, 91, 951-960.
General Stability
| General Stability | Organism |
|---|---|
| proteinase K is losing the proteolytic activity as the enzyme is attaining beta conformation | Parengyodontium album |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Parengyodontium album | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Keratin + H2O | Parengyodontium album | - |
? | - |
? |  |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| isopropanol | at 40% (v/v) isopropanol nearly half and at 50% (v/v) isopropanol all the tertiary structure is lost, beyond 30% (v/v) isopropanol there is some perturbation in the secondary structure of the protein | Parengyodontium album |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parengyodontium album | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Parengyodontium album | ? | - |
? | |
| Keratin + H2O | - |
Parengyodontium album | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endopeptidase K | - |
Parengyodontium album |
| Proteinase K | - |
Parengyodontium album |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 66 | - |
thermal unfolding is cooperative at pH 7.0 with transition midpoint of 66°C | Parengyodontium album |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | proteinase K maintains structural integrity in the range of pH 3.0-7.0 | Parengyodontium album |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
the enzyme acquires partially unfolded conformation at pH 2.5 with lower activity | Parengyodontium album |
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