Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.64 extracted from

  • Liao, J.; Warmuth, M.K.; Govindarajan, S.; Ness, J.E.; Wang, R.P.; Gustafsson, C.; Minshull, J.
    Engineering proteinase K using machine learning and synthetic genes (2007), BMC Biotechnol., 7, 16.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis protein engineering approach for increasing activity and heat stability of proteinase K. Protein design algorithms that only require the testing of a small number of variants represent a significant step towards a generic, resource-optimized protein engineering process Parengyodontium album

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding proteinase K re-synthesized with an Escherichia coli codon bias and cloned into an arabinose-inducible Escherichia coli expression vector Parengyodontium album

Protein Variants

Protein Variants Comment Organism
A199S no effect on activity Parengyodontium album
A236V lethal Parengyodontium album
E138A lethal, increased stability of the serine protease subtilisin Parengyodontium album
G293A strong positive effect on activity Parengyodontium album
I132V positive effect on activity Parengyodontium album
I310K no effect on activity, increased stability of the serine protease subtilisin Parengyodontium album
K208H positive effect on activity Parengyodontium album
K332R positive effect on activity Parengyodontium album
L180I positive effect on activity Parengyodontium album
L299C lethal, increased stability of the serine protease subtilisin Parengyodontium album
M145F no effect on activity, increased stability of the serine protease subtilisin Parengyodontium album
N95C lethal, increased stability of the serine protease subtilisin Parengyodontium album
P265S no effect on activity, increased stability of the serine protease subtilisin Parengyodontium album
P355S no effect on activity, increased stability of the serine protease subtilisin Parengyodontium album
P97S lethal, increased stability of the serine protease subtilisin Parengyodontium album
R237N no effect on activity Parengyodontium album
S107D no effect on activity Parengyodontium album
S123A positive effect on activity Parengyodontium album
S273T positive effect on activity Parengyodontium album
S337N positive effect on activity Parengyodontium album
V167I no effect on activity Parengyodontium album
V267I positive effect on activity Parengyodontium album
Y151A strong positive effect on activity Parengyodontium album
Y194S no effect on activity, random mutation obtained during synthesis of wild-type proteinase K Parengyodontium album

Organism

Organism UniProt Comment Textmining
Parengyodontium album
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutants purified over a Ni-NTA column Parengyodontium album

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
Parengyodontium album N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
Proteinase K
-
Parengyodontium album