Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme without signal peptide in Pichia pastoris strain KM71 | Aspergillus sojae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 15% inhibition at 10 mM | Aspergillus sojae | |
Fe2+ | 47% inhibition at 5 mM | Aspergillus sojae | |
leupeptin | 48% inhibition at 10 mM | Aspergillus sojae | |
additional information | no inhibition by 5 mM Mg2+ | Aspergillus sojae | |
pepstatin | 4.8% inhibition at 1 mM | Aspergillus sojae | |
PMSF | 92% inhibition at 1 mM | Aspergillus sojae | |
SDS | 60% inhibition at 0.5 mM | Aspergillus sojae | |
Zn2+ | 64% inhibition at 5 mM | Aspergillus sojae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme contains a signal peptide | Aspergillus sojae | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sojae | A0A386IRJ1 | - |
- |
Aspergillus sojae GIM3.33 | A0A386IRJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Pichia pastoris strain KM71 by nickel affinity chromatography, ultrafiltration, and gel filtration | Aspergillus sojae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cultivation at inoculum concentration OD600 value 20.0 in 40 ml working volume (in 500 ml flasks), methanol addition (1.0% volume ratio), 0.02% biotin solution, and YNB primary concentration (13.0 g/l) results in a protease activity of 400.4 U/ml after induction for three days | Aspergillus sojae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Pro-Arg-4-nitroanilide + H2O | very low activity of 3.3% compared to Ala-Ala-Pro-Phe-4-nitroanilide | Aspergillus sojae | Ala-Ala-Pro-Arg + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Arg-4-nitroanilide + H2O | very low activity of 3.3% compared to Ala-Ala-Pro-Phe-4-nitroanilide | Aspergillus sojae GIM3.33 | Ala-Ala-Pro-Arg + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Asn-4-nitroanilide + H2O | very low activity of 1.1% compared to Ala-Ala-Pro-Phe-4-nitroanilide | Aspergillus sojae | Ala-Ala-Pro-Asn + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Asn-4-nitroanilide + H2O | very low activity of 1.1% compared to Ala-Ala-Pro-Phe-4-nitroanilide | Aspergillus sojae GIM3.33 | Ala-Ala-Pro-Asn + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Aspergillus sojae | Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Aspergillus sojae GIM3.33 | Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Bovine serum albumin + H2O | - |
Aspergillus sojae | ? | - |
? | |
Bovine serum albumin + H2O | - |
Aspergillus sojae GIM3.33 | ? | - |
? | |
additional information | the recombinan enzyme shows a broad substrate specificity for natural proteins (e.g. soy proteins) and synthetic peptide substrates, and prefers substrates at P1 position with large hydrophobic side-chain groups. Ala-Ala-Pro-Glu-4-nitroanilide and glutin are a poor substrate | Aspergillus sojae | ? | - |
? | |
additional information | the recombinan enzyme shows a broad substrate specificity for natural proteins (e.g. soy proteins) and synthetic peptide substrates, and prefers substrates at P1 position with large hydrophobic side-chain groups. Ala-Ala-Pro-Glu-4-nitroanilide and glutin are a poor substrate | Aspergillus sojae GIM3.33 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41318, recombinant His6-tagged enzyme without signal peptide, sequence calculation, x * 35200, recombinant His6-tagged enzyme without signal peptide, SDS-PAGE | Aspergillus sojae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus sojae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, 60 min, over 50% activity remaining | Aspergillus sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Aspergillus sojae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | purified recombinant enzyme, stable at | Aspergillus sojae |
General Information | Comment | Organism |
---|---|---|
additional information | compared to papain (8.7%) and alcalase (12.2%), the degree of hydrolysis of rAp to soy protein isolate is 16.5% | Aspergillus sojae |