Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 94% activation at 1 mM, 70% at 5 mM | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | loss of 64-74% activity at 15-50% v/v after 24 h, loss of 27-70% activity after 1 h | Bacillus licheniformis | |
Ag+ | about 19% inhibition at 1-10 mM | Bacillus licheniformis | |
butanol | loss of 40-68% activity at 15-50% v/v after 24 h, loss of 40-47% activity after 1 h | Bacillus licheniformis | |
chloroform | loss of 45-88.5% activity at 15-50% v/v after 24 h, loss of 31-21% activity after 1 h | Bacillus licheniformis | |
Co2+ | 8% inhibition at 10 mM | Bacillus licheniformis | |
DMSO | loss of 41-53% activity at 15-50% v/v after 24 h, loss of 21-48% activity after 1 h | Bacillus licheniformis | |
DTNB | 52-57% inhibition at 1-5 mM | Bacillus licheniformis | |
EDTA | complete inhibition at 5 mM, 50% at 1 mM | Bacillus licheniformis | |
ethanol | loss of 55-78% activity at 15-50% v/v after 24 h, loss of 8-55% activity after 1 h | Bacillus licheniformis | |
Fe2+ | 45% inhibition at 10 mM, 33.5% at 5 mM | Bacillus licheniformis | |
formaldehyde | loss of 27-13% activity at 15-50% v/v after 24 h, loss of 46-47% activity after 1 h | Bacillus licheniformis | |
H2O2 | 73-74% inhibition at 1-5 mM | Bacillus licheniformis | |
Isopropanol | loss of 39-45% activity at 15-50% v/v after 24 h, loss of about 50% activity after 1 h | Bacillus licheniformis | |
methanol | loss of 75-100% activity at 15-50% v/v after 24 h, loss of 44-93% activity after 1 h | Bacillus licheniformis | |
Ni2+ | 32% inhibition at 10 mM, 28% at 5 mM | Bacillus licheniformis | |
PMSF | complete inhibition at 5 mM, 97% at 1 mM | Bacillus licheniformis | |
SDS | complete inhibition at 5 mM, 96% at 1 mM | Bacillus licheniformis | |
Triton X-100 | 45% inhibition at 5 mM, 39% at 1 mM | Bacillus licheniformis | |
Tween 20 | 66% inhibition at 5 mM, 39% at 1 mM | Bacillus licheniformis | |
Tween 80 | 72% inhibition at 1-5 mM | Bacillus licheniformis | |
Urea | 71% inhibition at 1 mM, 95.5% at 8 mM | Bacillus licheniformis | |
Zn2+ | 40% inhibition at 10 mM, 38% at 1-5 mM | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km and Vmax values of the protease enzyme purified from strain A10 are 0.033 mg/ml and 8.17 mmol/ml/min, respectively, with casein as substrate | Bacillus licheniformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus licheniformis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates 15% at 10 mM | Bacillus licheniformis | |
Mg2+ | activates 5% at 10 mM | Bacillus licheniformis | |
Mn2+ | activates 14.5% at 10 mM | Bacillus licheniformis | |
additional information | no effect on activity by Ca2+ | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
originally isolated from Erzurum Ilica hot springs | - |
Bacillus licheniformis A10 | - |
originally isolated from Erzurum Ilica hot springs | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | 97% activity compared to casein | Bacillus licheniformis | ? | - |
? | |
azocasein + H2O | 97% activity compared to casein | Bacillus licheniformis A10 | ? | - |
? | |
Bovine serum albumin + H2O | 18% activity compared to casein | Bacillus licheniformis | ? | - |
? | |
Bovine serum albumin + H2O | 18% activity compared to casein | Bacillus licheniformis A10 | ? | - |
? | |
casein + H2O | the enzyme has a high affinity for the casein, best substrate | Bacillus licheniformis | ? | - |
? | |
casein + H2O | the enzyme has a high affinity for the casein, best substrate | Bacillus licheniformis A10 | ? | - |
? | |
Gelatin + H2O | 28% activity compared to casein | Bacillus licheniformis | ? | - |
? | |
Gelatin + H2O | 28% activity compared to casein | Bacillus licheniformis A10 | ? | - |
? | |
Hemoglobin + H2O | 60% activity compared to casein | Bacillus licheniformis | ? | - |
? | |
Hemoglobin + H2O | 60% activity compared to casein | Bacillus licheniformis A10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40550, extracellular enzyme, SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
Alkaline protease | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | over 70% of maximal activity within this range, profile overview | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | purified native extracellular enzyme, over 50% activity remaining after 15-30 min. After 60-min incubation, the enzyme preserves 70.74%, 54.22%, 94.82%, and 98.47% of its activity at 40°C, 50°C, 60°C, and 70°C, respectively | Bacillus licheniformis |
60 | 70 | purified native extracellular enzyme, 1 h, 90% activity remaining | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.392 | - |
casein | pH 8.0, 37°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | activity range, 70% of maximal activity at pH 6.0, 60% at pH 11.0, profile overview | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
purified native extracellular enzyme, 40% of activity remaining | Bacillus licheniformis |
7.5 | 10.5 | purified native extracellular enzyme, over 60% activity remianing within this range | Bacillus licheniformis |
11 | - |
purified native extracellular enzyme, 30% of activity remaining | Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a serine protease | Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.392 | - |
casein | pH 8.0, 37°C | Bacillus licheniformis |