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Literature summary for 3.4.21.63 extracted from

  • Salihi, A.; Asoodeh, A.; Aliabadian, M.
    Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93 (2017), Int. J. Biol. Macromol., 94, 827-835 .
    View publication on PubMed
Search for more literature for 3.4.21.63

Activating Compound

Activating Compound Comment Organism Structure
acetone activates 2fold at 30% v/v Aspergillus oryzae
hexane activates slightly at 15% v/v Aspergillus oryzae
isoamyl alcohol activates 1.5fold at 30% v/v Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Aspergillus oryzae
2-propanol inhibits at 30% v/v Aspergillus oryzae
cetyltrimethyl ammonium bromide
-
 Aspergillus oryzae
DMSO inhibits at 30% v/v Aspergillus oryzae
EDTA
-
 Aspergillus oryzae
H2O2
-
 Aspergillus oryzae
Isopropanol inhibits at 30% v/v Aspergillus oryzae
additional information Triton X-100 and PMSF have no inhibitory effect on the enzyme activity Aspergillus oryzae
SDS
-
 Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information casein gives a Vmax of 141.1 ng/min and a Km of 2432 ng/ml Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 19% activation at 5 mM, no activation at 2 mM Aspergillus oryzae
Fe2+ 13% activation at 2 mM, 40% at 5 mM Aspergillus oryzae
Mg2+ 20% activation at 5 mM, no activation at 2 mM Aspergillus oryzae
Mn2+ 21% activation at 2 mM, 14% at 5 mM Aspergillus oryzae
additional information no significant effect with Mn2+ and Cu2+ at 2-5 mM Aspergillus oryzae
Na+ 14% activation at 2 mM, no activation at 5 mM Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran
-
Aspergillus oryzae CH93
-
 isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium cultivation on solid medium, optimization of culture conditions, overview Aspergillus oryzae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Aspergillus oryzae ?
-
 ?
azocasein + H2O
-
 Aspergillus oryzae CH93 ?
-
 ?
Bovine serum albumin + H2O
-
 Aspergillus oryzae ?
-
 ?
Bovine serum albumin + H2O
-
 Aspergillus oryzae CH93 ?
-
 ?
casein + H2O best substrate Aspergillus oryzae ?
-
 ?
casein + H2O best substrate Aspergillus oryzae CH93 ?
-
 ?
Gelatin + H2O low activity Aspergillus oryzae ?
-
 ?
Gelatin + H2O low activity Aspergillus oryzae CH93 ?
-
 ?
N-acetyl-L-tyrosine ethylester + H2O low activity Aspergillus oryzae ?
-
 ?
N-acetyl-L-tyrosine ethylester + H2O low activity Aspergillus oryzae CH93 ?
-
 ?

Synonyms

Synonyms Comment Organism
Alkaline protease
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, half-life is 100 min Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Aspergillus oryzae