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Literature summary for 3.4.21.63 extracted from

  • Niyonzima, F.N.; More, S.S.
    Purification and characterization of detergent-compatible protease from Aspergillus terreus gr (2015), 3 Biotech, 5, 61-70 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by H2O2 and NaClO Aspergillus terreus
SDS
-
Aspergillus terreus
Triton X-100
-
Aspergillus terreus
Tween 80
-
Aspergillus terreus

Application

Application Comment Organism
detergent alkaline proteases are primarily used in the detergent industry. The addition of the protease to the detergent Super wheel improved its blood stain removal Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
DTT 9.9% inhibition at 5 mM Aspergillus terreus
EDTA 12.7% inhibition at 5 mM Aspergillus terreus
Fe3+ 18.9% inhibition at 5 mM Aspergillus terreus
Hg2+ 25.2% inhibition at 5 mM Aspergillus terreus
iodoacetamide 5.2% inhibition at 5 mM Aspergillus terreus
Mn2+ 14.1% inhibition at 5 mM Aspergillus terreus
additional information no effect by H2O2 and NaClO Aspergillus terreus
N-bromosuccinimide 50.3% inhibition at 5 mM Aspergillus terreus
N-diazoacetylnorleucine methyl ester 8.3% inhibition at 5 mM Aspergillus terreus
N-ethylmaleimide 11.2% inhibition at 5 mM Aspergillus terreus
Na+ 7.5% inhibition at 5 mM Aspergillus terreus
PMSF 83.7% inhibition at 5 mM Aspergillus terreus
Sodium azide 22.0% inhibition at 5 mM Aspergillus terreus
tosyl-L-lysylchloromethyl ketone 6.7% inhibition at 5 mM Aspergillus terreus
Urea 4.5% inhibition at 5 mM Aspergillus terreus
Zn2+ 10.9% inhibition at 5 mM Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for casein is 5.4 mg/ml Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 82.6% at 10 mM Aspergillus terreus
Co2+ activates 21.1% at 10 mM Aspergillus terreus
Mg2+ activates 52.2% at 10 mM Aspergillus terreus
additional information no or poor effects by 10 mM of K+, Ba2+, Cu2+, and Fe2+ Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
about, native PAGE Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography Aspergillus terreus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
148.9
-
purified enzyme, pH 11.0, 40°C Aspergillus terreus

Storage Stability

Storage Stability Organism
the enzyme exhibits high storage stability at 4°C, 28°C, and -20°C Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O
-
Aspergillus terreus ?
-
?
Bovine serum albumin + H2O
-
Aspergillus terreus gr. ?
-
?
casein + H2O best substrate Aspergillus terreus ?
-
?
casein + H2O best substrate Aspergillus terreus gr. ?
-
?
Gelatin + H2O the active enzyme degrades the copolymerized gelatin with high activity Aspergillus terreus ?
-
?
Gelatin + H2O the active enzyme degrades the copolymerized gelatin with high activity Aspergillus terreus gr. ?
-
?
hen egg albumin + H2O
-
Aspergillus terreus ?
-
?
hen egg albumin + H2O
-
Aspergillus terreus gr. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 16240, SDS-PAGE and LC-MS Aspergillus terreus

Synonyms

Synonyms Comment Organism
Alkaline protease
-
Aspergillus terreus
Aspergillus terreus protease
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 purified enzyme, 24 h, completely stable at Aspergillus terreus
60 90 purified secreted enzyme, 1 h, completely stable at Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
11 12 purified enzyme, 28°C, 24 h, completely stable at Aspergillus terreus