BRENDA - Enzyme Database
show all sequences of 3.4.21.63

Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates

te Biesebeke, R.; Record, E.; van Biezen, N.; Heerikhuisen, M.; Franken, A.; Punt, P.J.; van den Hondel, C.A.; Appl. Microbiol. Biotechnol. 69, 44-50 (2005)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
additional information
enzyme disruption mutant, strain shows much more compact colony morphology than wild-type. Sporulation occurs two days later, and hyphal growth and length is on average 50% of that of wild-type, with increased hyphal branching. After growth on wheat kernels, mutant strain produces at least 50% higher amylase, 100% higher glucoamylase and 90% higher protease activity levels than wild-type
Aspergillus oryzae
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus oryzae
-
pclA gene product
-
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
enzyme disruption mutant, strain shows much more compact colony morphology than wild-type. Sporulation occurs two days later, and hyphal growth and length is on average 50% of that of wild-type, with increased hyphal branching. After growth on wheat kernels, mutant strain produces at least 50% higher amylase, 100% higher glucoamylase and 90% higher protease activity levels than wild-type
Aspergillus oryzae
Other publictions for EC 3.4.21.63
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
754496
Gao
Separation, biochemical chara ...
Aspergillus oryzae 3.042, Aspergillus oryzae
J. Sci. Food. Agric.
99
3359-3366
2019
-
1
-
-
-
-
6
1
-
4
-
-
-
2
-
-
1
-
-
-
1
-
-
2
1
1
1
3
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
2
1
1
3
-
1
1
1
-
-
1
1
-
-
-
753838
Soares da Silva
Thermodynamic investigation o ...
Aspergillus tamarii, Aspergillus tamarii URM4634
Int. J. Biol. Macromol.
109
1039-1044
2018
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
1
1
-
1
-
1
-
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1
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1
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2
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1
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1
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1
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-
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-
-
-
-
-
-
753842
Abdel Wahab
Response surface methodology ...
Aspergillus niger, Aspergillus niger WA 2017
Int. J. Biol. Macromol.
115
447-458
2018
2
1
-
-
-
-
8
-
-
1
-
-
3
2
-
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-
-
1
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2
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1
1
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1
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1
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1
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2
1
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8
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1
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3
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1
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2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
755309
Ke
High-level expression, purifi ...
Aspergillus sojae, Aspergillus sojae GIM3.33
Protein Expr. Purif.
148
24-29
2018
-
-
1
-
-
-
8
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
10
1
-
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
1
-
-
-
-
-
-
1
-
1
-
-
10
1
1
-
1
-
1
-
1
-
-
1
1
-
-
-
753873
Salihi
Production and biochemical ch ...
Aspergillus oryzae, Aspergillus oryzae CH93
Int. J. Biol. Macromol.
94
827-835
2017
3
-
-
-
-
-
9
1
1
6
-
-
-
2
-
-
1
-
-
1
-
-
10
-
1
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
9
-
1
1
6
-
-
-
-
-
1
-
1
-
-
10
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
755225
Ida
Biochemical properties and ev ...
Aspergillus fischeri, Aspergillus fischeri ATCC 1020, Aspergillus fischeri CBS 544.65, Aspergillus fischeri DSM 3700, Aspergillus fischeri FGSC A1164, Aspergillus fischeri JCM 1740, Aspergillus fischeri WB 181, Penicillium citrinum
Prep. Biochem. Biotechnol.
47
282-290
2017
-
4
-
-
-
2
9
-
2
-
-
-
-
8
-
-
-
-
-
2
-
-
24
-
2
2
2
7
-
2
2
-
-
-
-
-
-
4
-
-
-
-
2
-
9
-
-
2
-
-
-
-
-
-
-
-
2
-
-
24
-
2
2
7
-
2
2
-
-
-
-
-
-
-
-
754276
Yilmaz
Thermotolerant alkaline prote ...
Bacillus licheniformis, Bacillus licheniformis A10
J. Enzyme Inhib. Med. Chem.
31
1241-1247
2016
1
-
-
-
-
-
22
1
1
4
-
-
-
2
-
-
1
-
-
-
-
-
10
1
1
1
1
2
1
1
1
3
-
-
-
-
1
-
-
-
-
-
-
-
22
-
1
1
4
-
-
-
-
-
1
-
-
-
-
10
1
1
1
2
1
1
1
3
-
-
1
1
-
1
1
732387
Selvam
Exoproteome of Aspergillus fla ...
Aspergillus flavus
J. Proteomics
115
23-35
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
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1
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3
1
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-
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1
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-
-
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-
-
1
-
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-
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1
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1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
752341
Niyonzima
Purification and characteriza ...
Aspergillus terreus
3 Biotech
5
61-70
2015
4
1
-
-
-
-
16
1
-
4
1
-
-
2
-
-
1
-
-
-
1
1
8
1
2
1
-
2
-
1
-
1
-
-
-
-
4
1
-
-
-
-
-
-
16
-
1
-
4
1
-
-
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-
1
-
-
1
1
8
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
754408
Xin
Purification and characteriza ...
Streptomyces sp. M30
J. Microbiol. Biotechnol.
25
1944-1953
2015
4
1
1
-
-
-
9
1
1
3
-
-
1
3
-
-
1
-
-
-
1
-
4
2
2
1
2
4
-
1
2
1
-
-
-
-
4
1
1
-
-
-
-
-
9
-
1
1
3
-
-
1
-
-
1
-
-
1
-
4
2
1
2
4
-
1
2
1
-
-
1
1
-
-
-
731156
Castro-Ochoa
Evaluation of strategies to im ...
Aspergillus nidulans, Aspergillus nidulans PW1
Appl. Biochem. Biotechnol.
169
1672-1682
2013
-
-
1
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
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1
3
1
-
-
-
1
-
-
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1
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1
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1
-
-
-
1
1
-
-
-
1
-
-
-
1
-
-
1
-
-
731567
Morais
-
Action of a pancreatin and an ...
Aspergillus oryzae
Braz. Arch. Biol. Technol.
56
985-995
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
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1
1
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1
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-
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-
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-
-
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-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
717111
Morya
In silico characterization of ...
Aspergillus clavatus, Aspergillus clavatus ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1, Aspergillus flavus, Aspergillus flavus ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167, Aspergillus oryzae, Aspergillus oryzae RIB 40
Appl. Biochem. Biotechnol.
166
243-257
2012
-
-
3
-
-
-
-
-
-
-
-
-
-
13
-
-
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4
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-
-
-
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731392
Syed
Functional analysis and struct ...
Aspergillus flavus
Bioinformation
8
175-180
2012
-
-
-
-
-
-
-
-
-
-
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1
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1
1
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1
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1
1
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-
-
731961
Ke
Enzymatic characteristics of a ...
Aspergillus oryzae
J. Agric. Food Chem.
60
12164-12169
2012
-
1
1
-
-
-
2
-
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1
-
-
-
6
-
-
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-
-
-
-
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2
-
2
1
-
1
-
1
-
1
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-
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1
1
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-
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-
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2
-
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1
-
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-
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-
-
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-
-
2
-
1
-
1
-
1
-
1
-
-
1
1
-
-
-
717055
Yadav
-
Oxidant and solvent stable alk ...
Aspergillus flavus, Aspergillus flavus MTCC 9952
Afr. J. Biotechnol.
10
8630-8640
2011
-
1
-
-
-
-
7
-
1
4
-
-
11
2
1
-
1
-
-
1
-
-
2
-
-
1
1
4
-
1
-
1
-
-
-
-
-
1
-
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-
-
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7
-
-
1
4
-
-
11
1
-
1
-
1
-
-
2
-
1
1
4
-
1
-
1
-
-
1
1
-
-
-
717658
Behnsen
Secreted Aspergillus fumigatus ...
Aspergillus fumigatus, Aspergillus fumigatus DELTAakuBKU80
Infect. Immun.
78
3585-3594
2010
-
-
1
-
1
-
-
-
1
-
-
2
-
5
-
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-
-
-
-
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2
-
1
1
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1
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1
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1
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1
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2
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-
-
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2
-
1
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1
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1
1
-
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682690
Guo
High-level expression, purific ...
Aspergillus oryzae, Aspergillus oryzae RIB 40
Protein Expr. Purif.
58
301-308
2008
-
-
1
-
-
-
9
-
-
2
1
-
-
3
-
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1
-
-
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2
-
2
1
2
1
1
1
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1
1
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1
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9
-
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2
1
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1
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2
-
2
1
1
1
1
-
1
1
-
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677898
Miyazawa
-
Use of Aspergillus oryzae prot ...
Aspergillus oryzae
Biocatal. Biotransform.
24
291-298
2006
-
-
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2
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678860
te Biesebeke
Expression of Aspergillus hemo ...
Aspergillus oryzae, Aspergillus oryzae ATCC 16168
Biotechnol. J.
1
822-827
2006
1
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2
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2
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1
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2
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679374
Pereira
Production and biochemical cha ...
Aspergillus fumigatus
Curr. Microbiol.
52
430-434
2006
1
-
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-
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2
-
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2
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1
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1
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1
3
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2
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1
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1
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680223
Babu
-
Response surface optimization ...
Aspergillus foetidus, Aspergillus foetidus NCIM637
Int. J. Chem. Sci.
4
951-958
2006
-
1
-
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2
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680260
Sharma
-
Partial purification of an alk ...
Aspergillus oryzae, Aspergillus oryzae AWT20
Internet J. Microbiol.
2
0000
2006
1
1
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-
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8
1
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2
1
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2
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1
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2
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2
1
1
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1
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1
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1
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1
1
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8
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1
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2
1
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1
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2
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2
1
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1
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1
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1
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667281
te Biesebeke
Branching mutants of Aspergill ...
Aspergillus oryzae
Appl. Microbiol. Biotechnol.
69
44-50
2005
-
-
-
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1
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1
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1
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671008
Tremacoldi
-
Production of extracellular al ...
Aspergillus clavatus
World J. Microbiol. Biotechnol.
21
169-172
2005
-
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-
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1
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1
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1
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3
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1
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1
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1
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1
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3
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The purification and activitie ...
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1
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1
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1
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1
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29488
Impoolsup
Isolation of alkaline and neut ...
Aspergillus flavus
Appl. Environ. Microbiol.
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1981
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2
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1
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1
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1
1
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1
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1
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1
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2
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1
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29492
Nakatani
Interaction of Asp. melleus Se ...
Aspergillus melleus
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1
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29491
Kundu
Purification and characterizat ...
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Appl. Microbiol.
30
507-513
1975
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1
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6
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1
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1
1
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1
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6
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1
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29482
Spadari
Highly restricted specificity ...
Aspergillus oryzae
Biochim. Biophys. Acta
359
267-272
1974
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2
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2
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29483
Morihara
Comparative study of various s ...
Aspergillus melleus, Aspergillus sojae
Arch. Biochem. Biophys.
165
72-79
1974
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2
13
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2
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30
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8
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2
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13
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30
-
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-
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8
-
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29498
Toepfer
Characterization of alkaline p ...
Aspergillus ochraceus
Folia Haematol. Int. Mag. Klin. Morphol. Blutforsch.
101
91-98
1974
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1
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1
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1
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4
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1
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1
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4
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29489
Nakadai
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Purification and properties of ...
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Agric. Biol. Chem.
37
2685-2694
1973
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3
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1
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1
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1
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1
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5
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5
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1
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3
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1
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1
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1
-
5
-
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-
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5
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1
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-
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29490
Danno
-
Substrate specificity of alkal ...
Aspergillus sulphureus, Aspergillus sydowii
Agric. Biol. Chem.
37
1493-1496
1973
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4
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4
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29494
Feinstein
Isolation of alkaline proteina ...
Aspergillus oryzae
Biochim. Biophys. Acta
309
196-202
1973
-
-
-
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3
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1
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1
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3
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3
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3
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1
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3
-
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3
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-
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-
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-
29495
Klapper
The purification and propertie ...
Aspergillus oryzae, Aspergillus oryzae NRRL 2160
Biochim. Biophys. Acta
304
505-512
1973
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-
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3
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1
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1
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1
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4
1
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2
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3
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1
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1
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1
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4
1
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-
2
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29497
Danno
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Inhibitory effect of alcohols ...
Aspergillus sulphureus
Agric. Biol. Chem.
37
445-446
1973
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4
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1
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4
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29481
Turkova
Alkaline proteinases of the ge ...
Aspergillus oryzae, Aspergillus sojae, Aspergillus sulphureus
Biochim. Biophys. Acta
257
257-263
1972
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-
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-
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3
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3
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3
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3
-
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-
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-
-
-
-
-
-
-
-
-
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3
-
-
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29485
Hayashi
-
Some characteristics of hydrol ...
Aspergillus sojae
Agric. Biol. Chem.
36
1755-1765
1972
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1
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10
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-
-
10
-
-
-
-
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-
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29486
Nasuno
-
Purification of alkaline prote ...
Aspergillus candidus
Agric. Biol. Chem.
36
1791-1796
1972
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2
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1
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1
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2
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2
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1
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-
1
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-
2
-
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-
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-
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29487
Ohara
-
Enzymic properties of alkaline ...
Aspergillus candidus
Agric. Biol. Chem.
36
1797-1802
1972
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-
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1
15
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1
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1
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-
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1
-
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3
-
1
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2
-
3
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-
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-
-
-
-
-
-
-
1
-
15
-
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1
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-
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-
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-
-
-
-
-
1
-
3
-
1
-
2
-
3
-
-
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29496
Ku
Alkaline protease from Aspergi ...
Aspergillus oryzae
Biochim. Biophys. Acta
268
225-232
1972
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1
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1
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1
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2
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-
1
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-
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1
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-
-
-
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1
-
2
-
-
-
-
-
1
-
-
-
-
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29499
Lai
-
Isolation, purification, and s ...
Aspergillus sojae, Aspergillus sojae NTU-163
J. Chin. Biochem. Soc.
1
61-71
1972
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1
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2
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1
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4
-
-
1
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-
1
-
-
-
-
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-
-
-
-
-
-
-
-
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1
-
-
-
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1
-
-
-
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4
-
1
-
-
-
1
-
-
-
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29500
Turkova
-
Reinvestigation of molecular w ...
Aspergillus flavus
Collect. Czech. Chem. Commun.
37
1408-1411
1972
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2
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1
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1
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-
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2
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29484
Nakagawa
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Alkaline proteinases from Aspe ...
Aspergillus flavus, Aspergillus oryzae, Aspergillus sojae, Aspergillus sydowii
Methods Enzymol.
19
581-591
1970
-
-
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-
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11
-
4
-
2
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4
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2
1
-
-
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2
-
4
-
-
-
-
2
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2
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1
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-
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11
-
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4
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2
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2
1
-
-
2
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4
-
-
-
2
-
2
-
1
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-
-
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-