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Literature summary for 3.4.21.63 extracted from

  • Klapper, B.F.; Jameson, D.M.; Mayer, R.M.
    The purification and properties of an extracellular protease from Aspergillus oryzae NRRL 2160 (1973), Biochim. Biophys. Acta, 304, 505-512.
    View publication on PubMed
Search for more literature for 3.4.21.63

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl acetate increasing concentrations of the ester inhibit casein hydrolysis Aspergillus oryzae
DFP
-
 Aspergillus oryzae
PMSF
-
 Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme produced in shake cultures is secreted in the stationary phase when cells are grown on nutrient broth, and in early growth on defined salts Aspergillus oryzae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 Aspergillus oryzae, gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 NRRL 2160
-
Aspergillus oryzae NRRL 2160
-
 NRRL 2160
-

Purification (Commentary)

Purification (Comment) Organism
NRRL 2160 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Aspergillus oryzae 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Aspergillus oryzae NRRL 2160 4-nitrophenol + acetate
-
 ?
casein + H2O
-
 Aspergillus oryzae hydrolyzed casein
-
 ?
casein + H2O
-
 Aspergillus oryzae NRRL 2160 hydrolyzed casein
-
 ?

Subunits

Subunits Comment Organism
More protein is not composed of subunits Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 N-carbobenzoxyglycine 4-nitrophenyl ester Aspergillus oryzae
8 8.5 casein Aspergillus oryzae